{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Lorenzo MF"],"funding":["Virginia Research Investment Fund","NCI NIH HHS","National Institutes of Health","NIGMS NIH HHS","NIH HHS"],"pagination":["1333"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8715747"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["14(12)"],"pubmed_abstract":["The treatment of CNS disorders suffers from the inability to deliver large therapeutic agents to the brain parenchyma due to protection from the blood-brain barrier (BBB). Herein, we investigated high-frequency pulsed electric field (HF-PEF) therapy of various pulse widths and interphase delays for BBB disruption while selectively minimizing cell ablation. Eighteen male Fisher rats underwent craniectomy procedures and two blunt-tipped electrodes were advanced into the brain for pulsing. BBB disruption was verified with contrast T1W MRI and pathologically with Evans blue dye. High-frequency irreversible electroporation cell death of healthy rodent astrocytes was investigated in vitro using a collagen hydrogel tissue mimic. Numerical analysis was conducted to determine the electric fields in"],"journal":["Pharmaceuticals (Basel, Switzerland)"],"pubmed_title":["An Investigation for Large Volume, Focal Blood-Brain Barrier Disruption with High-Frequency Pulsed Electric Fields."],"pmcid":["PMC8715747"],"funding_grant_id":["R25 GM072767","VRIF","P01CA207206","P01 CA207206","R01 CA213423"],"pubmed_authors":["Arroyo JP","Aycock KN","Rossmeisl JH","Lorenzo MF","Campelo SN","Hinckley J","Arena CB","Davalos RV"],"additional_accession":[]},"is_claimable":false,"name":"An Investigation for Large Volume, Focal Blood-Brain Barrier Disruption with High-Frequency Pulsed Electric Fields.","description":"The treatment of CNS disorders suffers from the inability to deliver large therapeutic agents to the brain parenchyma due to protection from the blood-brain barrier (BBB). Herein, we investigated high-frequency pulsed electric field (HF-PEF) therapy of various pulse widths and interphase delays for BBB disruption while selectively minimizing cell ablation. Eighteen male Fisher rats underwent craniectomy procedures and two blunt-tipped electrodes were advanced into the brain for pulsing. BBB disruption was verified with contrast T1W MRI and pathologically with Evans blue dye. High-frequency irreversible electroporation cell death of healthy rodent astrocytes was investigated in vitro using a collagen hydrogel tissue mimic. Numerical analysis was conducted to determine the electric fields in","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021 Dec","modification":"2026-04-08T10:30:08.497Z","creation":"2022-02-11T14:41:16.134Z"},"accession":"S-EPMC8715747","cross_references":{"pubmed":["34959733"],"doi":["10.3390/ph14121333"]}}