biostudies-literatureLorenzo MFVirginia Research Investment FundNCI NIH HHSNational Institutes of HealthNIGMS NIH HHSNIH HHS1333https://www.ebi.ac.uk/biostudies/studies/S-EPMC8715747biostudies-literatureUnknown14(12)The treatment of CNS disorders suffers from the inability to deliver large therapeutic agents to the brain parenchyma due to protection from the blood-brain barrier (BBB). Herein, we investigated high-frequency pulsed electric field (HF-PEF) therapy of various pulse widths and interphase delays for BBB disruption while selectively minimizing cell ablation. Eighteen male Fisher rats underwent craniectomy procedures and two blunt-tipped electrodes were advanced into the brain for pulsing. BBB disruption was verified with contrast T1W MRI and pathologically with Evans blue dye. High-frequency irreversible electroporation cell death of healthy rodent astrocytes was investigated in vitro using a collagen hydrogel tissue mimic. Numerical analysis was conducted to determine the electric fields inPharmaceuticals (Basel, Switzerland)An Investigation for Large Volume, Focal Blood-Brain Barrier Disruption with High-Frequency Pulsed Electric Fields.PMC8715747R25 GM072767VRIFP01CA207206P01 CA207206R01 CA213423Arroyo JPAycock KNRossmeisl JHLorenzo MFCampelo SNHinckley JArena CBDavalos RVfalseAn Investigation for Large Volume, Focal Blood-Brain Barrier Disruption with High-Frequency Pulsed Electric Fields.The treatment of CNS disorders suffers from the inability to deliver large therapeutic agents to the brain parenchyma due to protection from the blood-brain barrier (BBB). Herein, we investigated high-frequency pulsed electric field (HF-PEF) therapy of various pulse widths and interphase delays for BBB disruption while selectively minimizing cell ablation. Eighteen male Fisher rats underwent craniectomy procedures and two blunt-tipped electrodes were advanced into the brain for pulsing. BBB disruption was verified with contrast T1W MRI and pathologically with Evans blue dye. High-frequency irreversible electroporation cell death of healthy rodent astrocytes was investigated in vitro using a collagen hydrogel tissue mimic. Numerical analysis was conducted to determine the electric fields in2021-01-01T00:00:00Z2021 Dec2026-04-08T10:30:08.497Z2022-02-11T14:41:16.134ZS-EPMC87157473495973310.3390/ph14121333