{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["12(12)"],"submitter":["Maeno H"],"pubmed_abstract":["Serum is commonly used as a specimen in immunoassays but the presence of heterophilic antibodies can potentially interfere with the test results. Previously, we have developed a microfluidic device called: 3D Stack for enzyme-linked immunosorbent assay (ELISA). However, its evaluation was limited to detection from a single protein solution. Here, we investigated the sensitivity of the 3D Stack in detecting a severe dengue biomarker-soluble CD163 (sCD163)-within the serum matrix. To determine potential interactions with serum matrix, a spike-and-recovery assay was performed, using 3D Stacks with and without surface modification by an EDC-NHS (N-ethyl-N'-(3-(dimethylamino)propyl)carbodiimide/N-hydroxysuccinimide) coupling. Without surface modification, a reduced analyte recovery in proportio"],"journal":["Micromachines"],"pagination":["1503"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8715748"],"repository":["biostudies-literature"],"pubmed_title":["A 3D Microfluidic ELISA for the Detection of Severe Dengue: Sensitivity Improvement and Vroman Effect Amelioration by EDC-NHS Surface Modification."],"pmcid":["PMC8715748"],"pubmed_authors":["Wong PF","Mat Jelani A","Maeno H","Jamil-Abd J","Said RM","Mustafa M","Yang M","AbuBakar S","Sam SS","Shunmugarajoo A","Mageswaren E","Azmel A"],"additional_accession":[]},"is_claimable":false,"name":"A 3D Microfluidic ELISA for the Detection of Severe Dengue: Sensitivity Improvement and Vroman Effect Amelioration by EDC-NHS Surface Modification.","description":"Serum is commonly used as a specimen in immunoassays but the presence of heterophilic antibodies can potentially interfere with the test results. Previously, we have developed a microfluidic device called: 3D Stack for enzyme-linked immunosorbent assay (ELISA). However, its evaluation was limited to detection from a single protein solution. Here, we investigated the sensitivity of the 3D Stack in detecting a severe dengue biomarker-soluble CD163 (sCD163)-within the serum matrix. To determine potential interactions with serum matrix, a spike-and-recovery assay was performed, using 3D Stacks with and without surface modification by an EDC-NHS (N-ethyl-N'-(3-(dimethylamino)propyl)carbodiimide/N-hydroxysuccinimide) coupling. Without surface modification, a reduced analyte recovery in proportio","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021 Nov","modification":"2025-04-19T00:50:32.003Z","creation":"2022-02-11T14:28:37.495Z"},"accession":"S-EPMC8715748","cross_references":{"pubmed":["34945351"],"doi":["10.3390/mi12121503"]}}