biostudies-literatureLevack AENational Institute of Arthritis and Musculoskeletal and Skin DiseasesOrthopaedic Research and Education FoundationNIAMS NIH HHS1143-1153https://www.ebi.ac.uk/biostudies/studies/S-EPMC8716667biostudies-literatureUnknown40(5)There has been increasing interest in the use of a synthetic absorbable calcium sulfate (CaSO₄ ) for local antibiotic delivery in orthopaedic infections. The purpose of this study was to quantify elution kinetics of six antibiotics (amikacin, meropenem, fosfomycin, minocycline, cefazolin, and dalbavancin) from a clinically relevant CaSO₄ bead model and compare elution and antimicrobial activity to the current clinical gold standards: vancomycin and tobramycin. Antibiotic-loaded synthetic CaSO₄ beads were immersed in phosphate buffered saline and incubated at 37°C. Eluent was harvested at eight time points over 28 days. Antibiotic concentrations were measured by high performance liquid chromatography to quantify elution rates. CaSO₄ beads demonstrated burst release kinetics. Dalbavancin, cefazolin, and minocycline all demonstrated similar elution profiles to vancomycin. Amikacin and meropenem demonstrated favorable elution profiles and durations of above-minimum inhibitory concentration when compared to tobramycin. Clinical Significance: This study provides important novel data regarding the utility of amikacin, meropenem and dalbavancin as alternative choices to place in CaSO₄ carriers when treating orthopaedic infections.Journal of orthopaedic research : official publication of the Orthopaedic Research SocietyIdentifying alternative antibiotics that elute from calcium sulfate beads for treatment of orthopedic infections.PMC8716667T32 AR00728118‐070Yang XCarli AVMiller AOTurajane KLevack AEDriscoll DABostrom MPWellman DSfalseIdentifying alternative antibiotics that elute from calcium sulfate beads for treatment of orthopedic infections.There has been increasing interest in the use of a synthetic absorbable calcium sulfate (CaSO₄ ) for local antibiotic delivery in orthopaedic infections. The purpose of this study was to quantify elution kinetics of six antibiotics (amikacin, meropenem, fosfomycin, minocycline, cefazolin, and dalbavancin) from a clinically relevant CaSO₄ bead model and compare elution and antimicrobial activity to the current clinical gold standards: vancomycin and tobramycin. Antibiotic-loaded synthetic CaSO₄ beads were immersed in phosphate buffered saline and incubated at 37°C. Eluent was harvested at eight time points over 28 days. Antibiotic concentrations were measured by high performance liquid chromatography to quantify elution rates. CaSO₄ beads demonstrated burst release kinetics. Dalbavancin, cefazolin, and minocycline all demonstrated similar elution profiles to vancomycin. Amikacin and meropenem demonstrated favorable elution profiles and durations of above-minimum inhibitory concentration when compared to tobramycin. Clinical Significance: This study provides important novel data regarding the utility of amikacin, meropenem and dalbavancin as alternative choices to place in CaSO₄ carriers when treating orthopaedic infections.2022-01-01T00:00:00Z2022 May2024-11-20T01:22:08.596Z2024-11-20T01:22:08.596ZS-EPMC87166673419135010.1002/jor.25135