biostudies-literatureUnknown12Riaz SNorges ForskningsrådUniversitetet i BergenInnovationsfondenEfforts to develop broadly protective vaccines against pathogenic <i>Escherichia coli</i> are ongoing. A potential antigen candidate for vaccine development is the metalloprotease YghJ, or SslE. YghJ is a conserved mucinase that is immunogenic, heavily glycosylated, and produced by most pathogenic <i>E. coli</i>. To develop efficacious YghJ-based vaccines, there is a need to investigate to what extent potentially protective antibody responses target glycosylated epitopes in YghJ and to describe variations in the quality of YghJ glycosylation in the <i>E. coli</i> population. In this study we estimated the proportion of anti-YghJ IgA antibodies that targeted glycosylated epitopes in serum and intestinal lavage samples from 21 volunteers experimentally infected with wild-type enterotoxigenicFrontiers in immunology760135https://www.ebi.ac.uk/biostudies/studies/S-EPMC8718676biostudies-literatureCharacterization of Glycosylation-Specific Systemic and Mucosal IgA Antibody Responses to <i>Escherichia coli</i> Mucinase YghJ (SslE).PMC8718676Riaz SThorsing MSteinsland HAndersen AZHanevik KBoysen AfalseCharacterization of Glycosylation-Specific Systemic and Mucosal IgA Antibody Responses to <i>Escherichia coli</i> Mucinase YghJ (SslE).Efforts to develop broadly protective vaccines against pathogenic <i>Escherichia coli</i> are ongoing. A potential antigen candidate for vaccine development is the metalloprotease YghJ, or SslE. YghJ is a conserved mucinase that is immunogenic, heavily glycosylated, and produced by most pathogenic <i>E. coli</i>. To develop efficacious YghJ-based vaccines, there is a need to investigate to what extent potentially protective antibody responses target glycosylated epitopes in YghJ and to describe variations in the quality of YghJ glycosylation in the <i>E. coli</i> population. In this study we estimated the proportion of anti-YghJ IgA antibodies that targeted glycosylated epitopes in serum and intestinal lavage samples from 21 volunteers experimentally infected with wild-type enterotoxigenic2021-01-01T00:00:00Z20212026-07-16T15:33:51.678Z2022-02-11T14:49:38.865ZS-EPMC87186763497584910.3389/fimmu.2021.760135