biostudies-literatureUnknown1(11)Vogiazi VIntramural EPANational Science FoundationIn this study, we demonstrate the successful development of an electrochemical aptamer-based sensor for point-of-use detection and quantification of the highly potent microcystin-LR (MC-LR) in water. The sensor uses hexaammineruthenium(III) chloride ([Ru(NH3)6]3+) as redox mediator, because of the ability of the positively charged (3+) molecule to associate with the phosphate backbone of the nucleic acids. We quantitatively measure the target-induced displacement of aptamer associated, or surface confined, [Ru(NH3)6]3+ in the presence of MC-LR. Upon the addition of MC-LR in the water, surface-confined [Ru(NH3)6]3+ dissociates, resulting in less faradaic current from the reduction of [Ru(NH<suACS ES&T engineeringhttps://www.ebi.ac.uk/biostudies/studies/S-EPMC8722374biostudies-literatureSensitive Electrochemical Detection of Microcystin-LR in Water Samples Via Target-Induced Displacement of Aptamer Associated [Ru(NH3)6]3.PMC87223741706489EPA999999White RJVogiazi Vde la Cruz AVarughese EAHeineman WRDionysiou DDfalseSensitive Electrochemical Detection of Microcystin-LR in Water Samples Via Target-Induced Displacement of Aptamer Associated [Ru(NH3)6]3.In this study, we demonstrate the successful development of an electrochemical aptamer-based sensor for point-of-use detection and quantification of the highly potent microcystin-LR (MC-LR) in water. The sensor uses hexaammineruthenium(III) chloride ([Ru(NH3)6]3+) as redox mediator, because of the ability of the positively charged (3+) molecule to associate with the phosphate backbone of the nucleic acids. We quantitatively measure the target-induced displacement of aptamer associated, or surface confined, [Ru(NH3)6]3+ in the presence of MC-LR. Upon the addition of MC-LR in the water, surface-confined [Ru(NH3)6]3+ dissociates, resulting in less faradaic current from the reduction of [Ru(NH<su2021-01-01T00:00:00Z2021 Sep2025-04-04T19:40:21.956Z2025-04-04T19:40:21.956ZS-EPMC87223743498855110.1021/acsestengg.1c00256