biostudies-literatureUnknown12Wang SThe buffalo population is extensive in China, but its meat quality is relatively inferior. Therefore, improving meat quality should be one of the breeding goals. microRNAs (miRNAs) play an essential regulatory role in the post-transcriptional expression of genes. Some studies have reported their function regulating genes related to fat deposition and adipocyte differentiation in cattle, but there is limited reports in buffalo. We performed small RNA transcriptome sequencing of Xinyang buffalo adipose tissue between calves and adults in this study. As a result, 282 mature miRNAs were significantly differentially expressed, and co-expression analysis showed that 454 miRNAs were significantly associated with developmental stages. Target gene identification, GO (gene ontology) annotation, and Frontiers in genetics736441https://www.ebi.ac.uk/biostudies/studies/S-EPMC8727870biostudies-literatureIdentification and Functional Verification Reveals that miR-195 Inhibiting <i>THRSP</i> to Affect Fat Deposition in Xinyang Buffalo.PMC8727870Li HTang LPan CHuang JWang SMa XMa YWei XYang CfalseIdentification and Functional Verification Reveals that miR-195 Inhibiting <i>THRSP</i> to Affect Fat Deposition in Xinyang Buffalo.The buffalo population is extensive in China, but its meat quality is relatively inferior. Therefore, improving meat quality should be one of the breeding goals. microRNAs (miRNAs) play an essential regulatory role in the post-transcriptional expression of genes. Some studies have reported their function regulating genes related to fat deposition and adipocyte differentiation in cattle, but there is limited reports in buffalo. We performed small RNA transcriptome sequencing of Xinyang buffalo adipose tissue between calves and adults in this study. As a result, 282 mature miRNAs were significantly differentially expressed, and co-expression analysis showed that 454 miRNAs were significantly associated with developmental stages. Target gene identification, GO (gene ontology) annotation, and 2021-01-01T00:00:00Z20212026-06-20T03:20:03.821Z2022-02-11T15:06:28.458ZS-EPMC87278703500320510.3389/fgene.2021.736441