biostudies-literatureMeister JNational Institute of Diabetes and Digestive and Kidney DiseasesNational Institutes of HealthLundbeck FoundationNovo Nordisk Fonden101415https://www.ebi.ac.uk/biostudies/studies/S-EPMC8728399biostudies-literatureUnknown55<h4>Objective</h4>The goal of this study was to determine the glucometabolic effects of acute activation of G_s signaling in skeletal muscle (SKM) in vivo and its contribution to whole-body glucose homeostasis.<h4>Methods</h4>To address this question, we studied mice that express a G_s-coupled designer G protein-coupled receptor (Gs-DREADD or GsD) selectively in skeletal muscle. We also identified two G_s-coupled GPCRs that are endogenously expressed by SKM at relatively high levels (β₂-adrenergic receptor and CRF₂ receptor) and studied the acute metabolic effects of activating these receptors in vivo by highly selective agonists (clenbuterol and urocortin 2 (UCN2), respectively).<h4>Results</h4>Acute stimulation of GsD signaling in SKM iMolecular metabolismIn vivo metabolic effects after acute activation of skeletal muscle G_s signaling.PMC8728399NNF19OC0056839R313-2019-643Meister JLee RWess JJensen TELiu LBone DBJChen MBarella LFKleinert MKnudsen JRGavrilova OWeinstein LSfalseIn vivo metabolic effects after acute activation of skeletal muscle G_s signaling.<h4>Objective</h4>The goal of this study was to determine the glucometabolic effects of acute activation of G_s signaling in skeletal muscle (SKM) in vivo and its contribution to whole-body glucose homeostasis.<h4>Methods</h4>To address this question, we studied mice that express a G_s-coupled designer G protein-coupled receptor (Gs-DREADD or GsD) selectively in skeletal muscle. We also identified two G_s-coupled GPCRs that are endogenously expressed by SKM at relatively high levels (β₂-adrenergic receptor and CRF₂ receptor) and studied the acute metabolic effects of activating these receptors in vivo by highly selective agonists (clenbuterol and urocortin 2 (UCN2), respectively).<h4>Results</h4>Acute stimulation of GsD signaling in SKM i2022-01-01T00:00:00Z2022 Jan2026-05-28T05:19:33.402Z2022-02-11T15:08:16.856ZS-EPMC87283993488327810.1016/j.molmet.2021.101415