{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Liczner C"],"funding":["Natural Sciences and Engineering Research Council of Canada","Fonds de recherche du Québec – Nature et technologies"],"pagination":["410-420"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8729807"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["13(2)"],"pubmed_abstract":["A breadth of strategies are needed to efficiently modify oligonucleotides with peptides or lipids to capitalize on their therapeutic and diagnostic potential, including the modulation of <i>in vivo</i> chemical stability and for applications in cell-targeting and cell-permeability. The chemical linkages typically used in peptide oligonucleotide conjugates (POCs) have limitations in terms of stability and/or ease of synthesis. Herein, we report an efficient method for POC synthesis using a diselenide-selenoester ligation (DSL)-deselenization strategy that rapidly generates a stable amide linkage between the two biomolecules. This conjugation strategy is underpinned by a novel selenide phosphoramidite building block that can be incorporated into an oligonucleotide by solid-phase synthesis to"],"journal":["Chemical science"],"pubmed_title":["Generation of oligonucleotide conjugates <i>via</i> one-pot diselenide-selenoester ligation-deselenization/alkylation."],"pmcid":["PMC8729807"],"funding_grant_id":["RGPIN-2017-06683"],"pubmed_authors":["Liczner C","Hanna CC","Payne RJ","Wilds CJ"],"additional_accession":[]},"is_claimable":false,"name":"Generation of oligonucleotide conjugates <i>via</i> one-pot diselenide-selenoester ligation-deselenization/alkylation.","description":"A breadth of strategies are needed to efficiently modify oligonucleotides with peptides or lipids to capitalize on their therapeutic and diagnostic potential, including the modulation of <i>in vivo</i> chemical stability and for applications in cell-targeting and cell-permeability. The chemical linkages typically used in peptide oligonucleotide conjugates (POCs) have limitations in terms of stability and/or ease of synthesis. Herein, we report an efficient method for POC synthesis using a diselenide-selenoester ligation (DSL)-deselenization strategy that rapidly generates a stable amide linkage between the two biomolecules. This conjugation strategy is underpinned by a novel selenide phosphoramidite building block that can be incorporated into an oligonucleotide by solid-phase synthesis to","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Jan","modification":"2025-04-22T08:31:42.492Z","creation":"2025-04-05T22:33:39.597Z"},"accession":"S-EPMC8729807","cross_references":{"pubmed":["35126973"],"doi":["10.1039/d1sc04937b"]}}