biostudies-literaturede Carvalho CCCRResearch Unit iBB-Institute for Bioengineering and BiosciencesFCT in the scopeNIAID NIH HHSFundação para a Ciência e a Tecnologia, I.P. (FCT, Portugal) through programme ‘Investigador FCT 2013’National Institutes of HealthNIH HHS135-147https://www.ebi.ac.uk/biostudies/studies/S-EPMC8730685biostudies-literatureUnknown77(1)<h4>Background</h4>The cyclic anionic lipopeptide daptomycin is used in the treatment of severe infections caused by Gram-positive pathogens, including MRSA. Daptomycin resistance, although rare, often results in treatment failure. Paradoxically, in MRSA, daptomycin resistance is usually accompanied by a concomitant decrease in β-lactam resistance in what is known as the 'see-saw effect'. This resensitization is extensively used for the treatment of MRSA infections, by combining daptomycin and a β-lactam antibiotic, such as oxacillin.<h4>Objectives</h4>We aimed: (i) to investigate the combined effects of daptomycin and oxacillin on the lipid composition of the cellular membrane of both daptomycin-resistant and -susceptible MRSA strains; and (ii) to assess the involvement of the post-translThe Journal of antimicrobial chemotherapyImpact of PrsA on membrane lipid composition during daptomycin-resistance-mediated β-lactam sensitization in clinical MRSA strains.PMC8730685NIH-R56AI102503-01A1UIDB/04565/2020R56 AI102503IF/01203/ 2013/CP1163/CT0002UIDP/04565/2020Taglialegna ARosato AEde Carvalho CCCRfalseImpact of PrsA on membrane lipid composition during daptomycin-resistance-mediated β-lactam sensitization in clinical MRSA strains.<h4>Background</h4>The cyclic anionic lipopeptide daptomycin is used in the treatment of severe infections caused by Gram-positive pathogens, including MRSA. Daptomycin resistance, although rare, often results in treatment failure. Paradoxically, in MRSA, daptomycin resistance is usually accompanied by a concomitant decrease in β-lactam resistance in what is known as the 'see-saw effect'. This resensitization is extensively used for the treatment of MRSA infections, by combining daptomycin and a β-lactam antibiotic, such as oxacillin.<h4>Objectives</h4>We aimed: (i) to investigate the combined effects of daptomycin and oxacillin on the lipid composition of the cellular membrane of both daptomycin-resistant and -susceptible MRSA strains; and (ii) to assess the involvement of the post-transl2021-01-01T00:00:00Z2021 Dec2025-04-19T17:37:16.04Z2025-04-19T17:37:16.04ZS-EPMC87306853461803610.1093/jac/dkab356