biostudies-literatureHilverling ATechnische Universität DresdenDeutsche Forschungsgemeinschaft155-171https://www.ebi.ac.uk/biostudies/studies/S-EPMC8732932biostudies-literatureUnknown42(1)Autophagosome maturation comprises fusion with lysosomes and acidification. It is a critical step in the degradation of cytosolic protein aggregates that characterize many neurodegenerative diseases. In order to better understand this process, we studied intracellular trafficking of autophagosomes and aggregates of α-synuclein, which characterize Parkinson's disease and other synucleinopathies. The autophagosomal marker LC3 and the aggregation prone A53T mutant of α-synuclein were tagged by fluorescent proteins and expressed in HEK293T cells and primary astrocytes. The subcellular distribution and movement of these vesicle populations were analyzed by (time-lapse) microscopy. Fusion with lysosomes was assayed using the lysosomal marker LAMP1; vesicles with neutral and acidic luminal pH werCellular and molecular neurobiologyMaturing Autophagosomes are Transported Towards the Cell Periphery.PMC8732932FA-658/3-1Hilverling ASzego EMCozma DDinter ESaridaki TFalkenburger BHfalseMaturing Autophagosomes are Transported Towards the Cell Periphery.Autophagosome maturation comprises fusion with lysosomes and acidification. It is a critical step in the degradation of cytosolic protein aggregates that characterize many neurodegenerative diseases. In order to better understand this process, we studied intracellular trafficking of autophagosomes and aggregates of α-synuclein, which characterize Parkinson's disease and other synucleinopathies. The autophagosomal marker LC3 and the aggregation prone A53T mutant of α-synuclein were tagged by fluorescent proteins and expressed in HEK293T cells and primary astrocytes. The subcellular distribution and movement of these vesicle populations were analyzed by (time-lapse) microscopy. Fusion with lysosomes was assayed using the lysosomal marker LAMP1; vesicles with neutral and acidic luminal pH wer2022-01-01T00:00:00Z2022 Jan2026-04-12T15:45:49.772Z2022-02-11T15:23:43.118ZS-EPMC87329323410636110.1007/s10571-021-01116-0