biostudies-literatureLee HWministry of science and ict131-138https://www.ebi.ac.uk/biostudies/studies/S-EPMC8733102biostudies-literatureUnknown31(1)This study aimed to evaluate the virucidal effect and potential use as a disinfectant of undiluted and diluted slightly acidic electrolyzed water (SAEW) on human noroviruses (HuNoVs) using the in vitro suspension test and in food test. The oxidization reduction potential of SAEW gradually decreased with the increase in distilled water volume. Moreover, as the volume of distilled water and the dilution ratio increased, the available chlorine concentration of the samples significantly decreased from 31.2 ± 0.63 (SAEW) to 1.3 ± 0.21 (1:10 dilution of SAEW solution) (<i>p</i> < 0.05). Undiluted SAEW presented the lowest pH (5.56 ± 0.02) and as SAEW was diluted in distilled water, the pH of the sample increased. Considering the standard reduction values of pathogenic virus (> 4.00 log reductionFood science and biotechnologyPhysicochemical stability and virucidal effect of diluted, slightly acidic electrolyzed water against human norovirus.PMC8733102grant number KE2102-2Yang JSHa JHYoon SRPark BLee HWfalsePhysicochemical stability and virucidal effect of diluted, slightly acidic electrolyzed water against human norovirus.This study aimed to evaluate the virucidal effect and potential use as a disinfectant of undiluted and diluted slightly acidic electrolyzed water (SAEW) on human noroviruses (HuNoVs) using the in vitro suspension test and in food test. The oxidization reduction potential of SAEW gradually decreased with the increase in distilled water volume. Moreover, as the volume of distilled water and the dilution ratio increased, the available chlorine concentration of the samples significantly decreased from 31.2 ± 0.63 (SAEW) to 1.3 ± 0.21 (1:10 dilution of SAEW solution) (<i>p</i> < 0.05). Undiluted SAEW presented the lowest pH (5.56 ± 0.02) and as SAEW was diluted in distilled water, the pH of the sample increased. Considering the standard reduction values of pathogenic virus (> 4.00 log reduction2022-01-01T00:00:00Z2022 Jan2025-04-29T11:04:47.88Z2025-04-06T19:49:32.573ZS-EPMC87331023505923710.1007/s10068-021-01011-w