{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Lee CA"],"funding":["NIDA NIH HHS","National Institute on Drug Abuse"],"pagination":["809529"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8735849"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["12"],"pubmed_abstract":["Despite substantial research on neuronal circuits in nudipleuran gastropods, few peptides have been implicated in nudipleuran behavior. In this study, we expanded the understanding of peptides in this clade, using three species with well-studied nervous systems, <i>Hermissenda crassicornis</i>, <i>Melibe leonina</i>, and <i>Pleurobranchaea californica</i>. For each species, we performed sequence homology analysis of <i>de novo</i> transcriptome predictions to identify homologs to 34 of 36 prohormones previously characterized in the gastropods <i>Aplysia californica</i> and <i>Lymnaea stagnalis</i>. We then used single-cell mass spectrometry to characterize peptide profiles in homologous feeding interneurons: the multifunctional ventral white cell (VWC) in <i>P. californica</i> and the smal"],"journal":["Frontiers in physiology"],"pubmed_title":["Comparative Analysis of Neuropeptides in Homologous Interneurons and Prohormone Annotation in Nudipleuran Sea Slugs."],"pmcid":["PMC8735849"],"funding_grant_id":["P30 DA018310","P30DA018310"],"pubmed_authors":["Romanova EV","Gillette R","Southey BR","Sweedler JV","Lee CA"],"additional_accession":[]},"is_claimable":false,"name":"Comparative Analysis of Neuropeptides in Homologous Interneurons and Prohormone Annotation in Nudipleuran Sea Slugs.","description":"Despite substantial research on neuronal circuits in nudipleuran gastropods, few peptides have been implicated in nudipleuran behavior. In this study, we expanded the understanding of peptides in this clade, using three species with well-studied nervous systems, <i>Hermissenda crassicornis</i>, <i>Melibe leonina</i>, and <i>Pleurobranchaea californica</i>. For each species, we performed sequence homology analysis of <i>de novo</i> transcriptome predictions to identify homologs to 34 of 36 prohormones previously characterized in the gastropods <i>Aplysia californica</i> and <i>Lymnaea stagnalis</i>. We then used single-cell mass spectrometry to characterize peptide profiles in homologous feeding interneurons: the multifunctional ventral white cell (VWC) in <i>P. californica</i> and the smal","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021","modification":"2026-05-28T23:31:49.95Z","creation":"2022-02-11T15:10:38.209Z"},"accession":"S-EPMC8735849","cross_references":{"pubmed":["35002782"],"doi":["10.3389/fphys.2021.809529"]}}