{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["de Almeida SGC"],"funding":["UNESP-PROPe Research Office"],"pagination":["39"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8738833"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["12(1)"],"pubmed_abstract":["In this study, a mild-temperature two-step dilute acid and alkaline pretreatment (DA-AL) process was developed to generate highly digestible cellulose pulp from sugarcane bagasse for producing fermentable sugars by novel thermophilic cellulases derived from <i>Phomopsis stipata</i> SC 04. First, DA pretreatment of sugarcane bagasse at 2% (w/v) H<sub>2</sub>SO<sub>4</sub> and 121 °C for 71 min, followed by AL pretreatment at 2.2% (w/v) NaOH and 110 °C for 100 min led to the pulp containing 86% cellulose. The cellulose pulp was hydrolyzed by the immobilized <i>P. stipata</i> cellulase on Ca-alginate beads, following optimization of immobilization conditions. The results showed that mixing the cellulase extract and sodium alginate solutions at a volume ratio of 1:4 led to the highest immobili"],"journal":["3 Biotech"],"pubmed_title":["Saccharification of acid-alkali pretreated sugarcane bagasse using immobilized enzymes from <i>Phomopsis stipata</i>."],"pmcid":["PMC8738833"],"funding_grant_id":["09/2016-PROPe"],"pubmed_authors":["do Santos MG","Hassanpour M","Zhang Z","Giese EC","de Almeida SGC","de Mello GF","Dussan KJ","da Silva DDV"],"additional_accession":[]},"is_claimable":false,"name":"Saccharification of acid-alkali pretreated sugarcane bagasse using immobilized enzymes from <i>Phomopsis stipata</i>.","description":"In this study, a mild-temperature two-step dilute acid and alkaline pretreatment (DA-AL) process was developed to generate highly digestible cellulose pulp from sugarcane bagasse for producing fermentable sugars by novel thermophilic cellulases derived from <i>Phomopsis stipata</i> SC 04. First, DA pretreatment of sugarcane bagasse at 2% (w/v) H<sub>2</sub>SO<sub>4</sub> and 121 °C for 71 min, followed by AL pretreatment at 2.2% (w/v) NaOH and 110 °C for 100 min led to the pulp containing 86% cellulose. The cellulose pulp was hydrolyzed by the immobilized <i>P. stipata</i> cellulase on Ca-alginate beads, following optimization of immobilization conditions. The results showed that mixing the cellulase extract and sodium alginate solutions at a volume ratio of 1:4 led to the highest immobili","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Jan","modification":"2026-07-09T12:17:00.927Z","creation":"2025-04-06T09:43:55.587Z"},"accession":"S-EPMC8738833","cross_references":{"pubmed":["35070629"],"doi":["10.1007/s13205-021-03101-2"]}}