biostudies-literatureBennstein SBDeutsche Forschungsgemeinschaft797432https://www.ebi.ac.uk/biostudies/studies/S-EPMC8739490biostudies-literatureUnknown12Innate lymphoid cells (ILCs) and in particular ILC3s have been described to be vital for mucosal barrier functions and homeostasis within the gastrointestinal (GI) tract. Importantly, IL-22-secreting ILC3 have been implicated in the control of inflammatory bowel disease (IBD) and were shown to reduce the incidence of graft-versus-host disease (GvHD) as well as the risk of transplant rejection. Unfortunately, IL-22-secreting ILC3 are primarily located in mucosal tissues and are not found within the circulation, making access to them in humans challenging. On this account, there is a growing desire for clinically applicable protocols for <i>in vitro</i> generation of effector ILC3. Here, we present an approach for faithful generation of functionally competent human ILC3s from cord blood-deriFrontiers in immunologyEfficient <i>In Vitro</i> Generation of IL-22-Secreting ILC3 From CD34⁺ Hematopoietic Progenitors in a Human Mesenchymal Stem Cell Niche.PMC8739490SPP1937-UH91/8-1 (MU), FOR2033, TP B3 (RAJO)Weinhold SBennstein SBOostendorp RAJDegistirici ORaba KMeisel RWalter LKogler GUhrberg MfalseEfficient <i>In Vitro</i> Generation of IL-22-Secreting ILC3 From CD34⁺ Hematopoietic Progenitors in a Human Mesenchymal Stem Cell Niche.Innate lymphoid cells (ILCs) and in particular ILC3s have been described to be vital for mucosal barrier functions and homeostasis within the gastrointestinal (GI) tract. Importantly, IL-22-secreting ILC3 have been implicated in the control of inflammatory bowel disease (IBD) and were shown to reduce the incidence of graft-versus-host disease (GvHD) as well as the risk of transplant rejection. Unfortunately, IL-22-secreting ILC3 are primarily located in mucosal tissues and are not found within the circulation, making access to them in humans challenging. On this account, there is a growing desire for clinically applicable protocols for <i>in vitro</i> generation of effector ILC3. Here, we present an approach for faithful generation of functionally competent human ILC3s from cord blood-deri2021-01-01T00:00:00Z20212026-05-09T05:54:37.951Z2022-02-11T15:09:52.292ZS-EPMC87394903500312210.3389/fimmu.2021.797432