{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Saw JJ"],"funding":["National Aeronautics and Space Administration Astrobiology Institute","Mayo Nephrology/Urology Summer Undergraduate Research Fellowship","Mayo Clinic &amp; Illinois Alliance for Technology-Based Healthcare","O’Brien Urology Research Center"],"pagination":["298-311"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8740987"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["2(2)"],"pubmed_abstract":["<h4>Background</h4>Human kidney stones form <i>via</i> repeated events of mineral precipitation, partial dissolution, and reprecipitation, which are directly analogous to similar processes in other natural and manmade environments, where resident microbiomes strongly influence biomineralization. High-resolution microscopy and high-fidelity metagenomic (microscopy-to-omics) analyses, applicable to all forms of biomineralization, have been applied to assemble definitive evidence of <i>in vivo</i> microbiome entombment during urolithiasis.<h4>Methods</h4>Stone fragments were collected from a randomly chosen cohort of 20 patients using standard percutaneous nephrolithotomy (PCNL). Fourier transform infrared (FTIR) spectroscopy indicated that 18 of these patients were calcium oxalate (CaOx) sto"],"journal":["Kidney360"],"pubmed_title":["<i>In Vivo</i> Entombment of Bacteria and Fungi during Calcium Oxalate, Brushite, and Struvite Urolithiasis."],"pmcid":["PMC8740987"],"funding_grant_id":["DK101405","DK100227","NNA13AA91A"],"pubmed_authors":["Cregger MA","Merkel AC","Dong Y","Lieske JC","Krambeck AE","Lange D","Fields CJ","Sanford RA","Sivaguru M","Romero MF","Bruce WJ","Fouke BW","Rivera ME","Chia N","Saw JJ","Large T","Wilson EM","Bhattacharjee AS","Weber JR"],"additional_accession":[]},"is_claimable":false,"name":"<i>In Vivo</i> Entombment of Bacteria and Fungi during Calcium Oxalate, Brushite, and Struvite Urolithiasis.","description":"<h4>Background</h4>Human kidney stones form <i>via</i> repeated events of mineral precipitation, partial dissolution, and reprecipitation, which are directly analogous to similar processes in other natural and manmade environments, where resident microbiomes strongly influence biomineralization. High-resolution microscopy and high-fidelity metagenomic (microscopy-to-omics) analyses, applicable to all forms of biomineralization, have been applied to assemble definitive evidence of <i>in vivo</i> microbiome entombment during urolithiasis.<h4>Methods</h4>Stone fragments were collected from a randomly chosen cohort of 20 patients using standard percutaneous nephrolithotomy (PCNL). Fourier transform infrared (FTIR) spectroscopy indicated that 18 of these patients were calcium oxalate (CaOx) sto","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021 Feb","modification":"2025-04-22T09:14:19.558Z","creation":"2025-04-05T22:58:59.501Z"},"accession":"S-EPMC8740987","cross_references":{"pubmed":["35373025"],"doi":["10.34067/KID.0006942020","10.34067/kid.0006942020"]}}