{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Becerra D"],"funding":["NIAMS NIH HHS"],"pagination":["639-648"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8742269"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["27(12)"],"pubmed_abstract":["Lung regeneration is dependent on the availability of progenitor lung cells. Large numbers of self-renewing, patient-specific induced pluripotent stem cell-derived alveolar epithelial cells (iPSC-AECs) are needed to adequately recellularize whole-organ constructs. Prior methods to generate functional iPSC-AECs are not feasible for large-scale cell production. We present a novel protocol to produce iPSC-AECs, which is scalable for whole-organ regeneration. Differentiation of iPSCs was performed with genetically modified iPSCs with fluorescent reporters, which underwent differentiation in a stepwise protocol mimicking lung development. Cells were purified, sorted, and embedded in a liquid Matrigel precursor either to form adherent droplets or to form cell-laden Matrigel spheroids, which were"],"journal":["Tissue engineering. Part C, Methods"],"pubmed_title":["High-Throughput Culture Method of Induced Pluripotent Stem Cell-Derived Alveolar Epithelial Cells."],"pmcid":["PMC8742269"],"funding_grant_id":["P30 AR075042"],"pubmed_authors":["Becerra D","Ott HC","Wu T","Jeffs S"],"additional_accession":[]},"is_claimable":false,"name":"High-Throughput Culture Method of Induced Pluripotent Stem Cell-Derived Alveolar Epithelial Cells.","description":"Lung regeneration is dependent on the availability of progenitor lung cells. Large numbers of self-renewing, patient-specific induced pluripotent stem cell-derived alveolar epithelial cells (iPSC-AECs) are needed to adequately recellularize whole-organ constructs. Prior methods to generate functional iPSC-AECs are not feasible for large-scale cell production. We present a novel protocol to produce iPSC-AECs, which is scalable for whole-organ regeneration. Differentiation of iPSCs was performed with genetically modified iPSCs with fluorescent reporters, which underwent differentiation in a stepwise protocol mimicking lung development. Cells were purified, sorted, and embedded in a liquid Matrigel precursor either to form adherent droplets or to form cell-laden Matrigel spheroids, which were","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021 Dec","modification":"2025-04-04T19:38:12.982Z","creation":"2025-04-04T19:38:12.982Z"},"accession":"S-EPMC8742269","cross_references":{"pubmed":["34751582"],"doi":["10.1089/ten.tec.2021.0174","10.1089/ten.TEC.2021.0174"]}}