biostudies-literatureBecerra DNIAMS NIH HHS639-648https://www.ebi.ac.uk/biostudies/studies/S-EPMC8742269biostudies-literatureUnknown27(12)Lung regeneration is dependent on the availability of progenitor lung cells. Large numbers of self-renewing, patient-specific induced pluripotent stem cell-derived alveolar epithelial cells (iPSC-AECs) are needed to adequately recellularize whole-organ constructs. Prior methods to generate functional iPSC-AECs are not feasible for large-scale cell production. We present a novel protocol to produce iPSC-AECs, which is scalable for whole-organ regeneration. Differentiation of iPSCs was performed with genetically modified iPSCs with fluorescent reporters, which underwent differentiation in a stepwise protocol mimicking lung development. Cells were purified, sorted, and embedded in a liquid Matrigel precursor either to form adherent droplets or to form cell-laden Matrigel spheroids, which wereTissue engineering. Part C, MethodsHigh-Throughput Culture Method of Induced Pluripotent Stem Cell-Derived Alveolar Epithelial Cells.PMC8742269P30 AR075042Becerra DOtt HCWu TJeffs SfalseHigh-Throughput Culture Method of Induced Pluripotent Stem Cell-Derived Alveolar Epithelial Cells.Lung regeneration is dependent on the availability of progenitor lung cells. Large numbers of self-renewing, patient-specific induced pluripotent stem cell-derived alveolar epithelial cells (iPSC-AECs) are needed to adequately recellularize whole-organ constructs. Prior methods to generate functional iPSC-AECs are not feasible for large-scale cell production. We present a novel protocol to produce iPSC-AECs, which is scalable for whole-organ regeneration. Differentiation of iPSCs was performed with genetically modified iPSCs with fluorescent reporters, which underwent differentiation in a stepwise protocol mimicking lung development. Cells were purified, sorted, and embedded in a liquid Matrigel precursor either to form adherent droplets or to form cell-laden Matrigel spheroids, which were2021-01-01T00:00:00Z2021 Dec2025-04-04T19:38:12.982Z2025-04-04T19:38:12.982ZS-EPMC87422693475158210.1089/ten.tec.2021.017410.1089/ten.TEC.2021.0174