biostudies-literatureKupsco ANIEHS NIH HHS106986https://www.ebi.ac.uk/biostudies/studies/S-EPMC8742869biostudies-literatureUnknown158<h4>Background/aims</h4>Early life exposures to marine contaminants can adversely impact child health but modes of action are unclear. Human milk contains extracellular vesicles (EVs) that can transport biologically relevant cargo from mother to infant, including microRNAs (miRNAs), and may partly mediate the effects of pollutants on child health. However, the role of marine pollutants on miRNA expression in milk EVs is unexplored.<h4>Methods</h4>We isolated EV RNA from 333 milk samples collected between 2 and 74 days postpartum from a Faroese birth cohort born 1997-2000 and sequenced 2083 miRNAs using a targeted library preparation method. We quantified five perfluoroalkyl substances (PFAS), pesticide metabolite p,p'-dichlorodiphenyldichloroethylene (DDE), and the sum of three major polycEnvironment internationalMarine pollutant exposures and human milk extracellular vesicle-microRNAs in a mother-infant cohort from the Faroe Islands.PMC8742869R21 ES027087K99 ES030749R00 ES030749R21 ES029328P30 ES009089P42 ES027706T32 ES007142Prada DWeihe PKupsco ALee JJPetersen MSCoull BABaccarelli AAHu LGrandjean PValvi DfalseMarine pollutant exposures and human milk extracellular vesicle-microRNAs in a mother-infant cohort from the Faroe Islands.<h4>Background/aims</h4>Early life exposures to marine contaminants can adversely impact child health but modes of action are unclear. Human milk contains extracellular vesicles (EVs) that can transport biologically relevant cargo from mother to infant, including microRNAs (miRNAs), and may partly mediate the effects of pollutants on child health. However, the role of marine pollutants on miRNA expression in milk EVs is unexplored.<h4>Methods</h4>We isolated EV RNA from 333 milk samples collected between 2 and 74 days postpartum from a Faroese birth cohort born 1997-2000 and sequenced 2083 miRNAs using a targeted library preparation method. We quantified five perfluoroalkyl substances (PFAS), pesticide metabolite p,p'-dichlorodiphenyldichloroethylene (DDE), and the sum of three major polyc2022-01-01T00:00:00Z2022 Jan2026-05-09T22:27:55.146Z2025-02-18T22:34:40.504ZS-EPMC87428693499124810.1016/j.envint.2021.106986