{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Ziff OJ"],"funding":["Winton Group Leader in recognition of the Winton Charitable Foundation&apos;s","Cancer Research UK","Francis Crick Institute","MRC eMedLab Medical Bioinformatics Infrastructure","Ministry of higher education of Egypt","Medical Research Council","The Francis Crick Institute","Motor Neurone Disease Association","University College London Hospitals Biomedical Research Centre","Wellcome Trust"],"pagination":["71-84"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8744676"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["32(1)"],"pubmed_abstract":["Astrocytes contribute to motor neuron death in amyotrophic lateral sclerosis (ALS), but whether they adopt deleterious features consistent with inflammatory reactive states remains incompletely resolved. To identify inflammatory reactive features in ALS human induced pluripotent stem cell (hiPSC)-derived astrocytes, we examined transcriptomics, proteomics, and glutamate uptake in <i>VCP</i>-mutant astrocytes. We complemented this by examining other ALS mutations and models using a systematic meta-analysis of all publicly-available ALS astrocyte sequencing data, which included hiPSC-derived astrocytes carrying <i>SOD1</i>, <i>C9orf72</i>, and <i>FUS</i> gene mutations as well as mouse ALS astrocyte models with <i>SOD1<sup>G93A</sup></i> mutation, <i>Tardbp</i> deletion, and <i>Tmem259</i> ("],"journal":["Genome research"],"pubmed_title":["Meta-analysis of human and mouse ALS astrocytes reveals multi-omic signatures of inflammatory reactive states."],"pmcid":["PMC8744676"],"funding_grant_id":["MR/L016311/1","101149/Z/13/Z","Patani/Nov18/950-795","10110","MR/S006591/1","BRC689/ED/CB/100130","MR/M02492X/1","FC010110","103760/Z/14/Z"],"pubmed_authors":["Clarke BE","Taha DM","Ziff OJ","Crerar H","Luscombe NM","Patani R"],"additional_accession":[]},"is_claimable":false,"name":"Meta-analysis of human and mouse ALS astrocytes reveals multi-omic signatures of inflammatory reactive states.","description":"Astrocytes contribute to motor neuron death in amyotrophic lateral sclerosis (ALS), but whether they adopt deleterious features consistent with inflammatory reactive states remains incompletely resolved. To identify inflammatory reactive features in ALS human induced pluripotent stem cell (hiPSC)-derived astrocytes, we examined transcriptomics, proteomics, and glutamate uptake in <i>VCP</i>-mutant astrocytes. We complemented this by examining other ALS mutations and models using a systematic meta-analysis of all publicly-available ALS astrocyte sequencing data, which included hiPSC-derived astrocytes carrying <i>SOD1</i>, <i>C9orf72</i>, and <i>FUS</i> gene mutations as well as mouse ALS astrocyte models with <i>SOD1<sup>G93A</sup></i> mutation, <i>Tardbp</i> deletion, and <i>Tmem259</i> (","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Jan","modification":"2026-05-08T22:15:42.833Z","creation":"2022-02-11T16:11:34.836Z"},"accession":"S-EPMC8744676","cross_references":{"pubmed":["34963663"],"doi":["10.1101/gr.275939.121"]}}