{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Wise SA"],"funding":["Intramural NIH HHS","NIH Office of the Director"],"pagination":["351-366"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8748290"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["414(1)"],"pubmed_abstract":["An interlaboratory comparison study was conducted by the Vitamin D Standardization Program (VDSP) to assess the performance of ligand binding assays (Part 2) for the determination of serum total 25-hydroxyvitamin D [25(OH)D]. Fifty single-donor samples were assigned target values for concentrations of 25-hydroxyvitamin D<sub>2</sub> [25(OH)D<sub>2</sub>], 25-hydroxyvitamin D<sub>3</sub> [25(OH)D<sub>3</sub>], 3-epi-25-hydroxyvitamin D<sub>3</sub> [3-epi-25(OH)D<sub>3</sub>], and 24R,25-dihydroxyvitamin D<sub>3</sub> [24R,25(OH)<sub>2</sub>D<sub>3</sub>] using isotope dilution liquid chromatography-tandem mass spectrometry (ID LC-MS/MS). VDSP Intercomparison Study 2 Part 2 includes results from 17 laboratories using 32 ligand binding assays. Assay performance was evaluated using mean % bias"],"journal":["Analytical and bioanalytical chemistry"],"pubmed_title":["Interlaboratory comparison of 25-hydroxyvitamin D assays: Vitamin D Standardization Program (VDSP) Intercomparison Study 2 - Part 2 ligand binding assays - impact of 25-hydroxyvitamin D<sub>2</sub> and 24R,25-dihydroxyvitamin D<sub>3</sub> on assay performance."],"pmcid":["PMC8748290"],"funding_grant_id":["not applicable","Z99 OD999999"],"pubmed_authors":["Beckert C","Camara JE","Sempos CT","Pham H","Simpson C","Wise SA","Khan DA","Williams EL","Cavalier E","Batista MC","Nalin F","Schultess J","Parker N","Popp C","Lukas P","Kuszak AJ","Tourneur C","Kilbane M","Bennett A","Freeman J","Hahm G","Jansen E","Burdette CQ","Merkel J","Mushtaq S","Van Slooten G","Villarreal A","Durazo-Arvizu RA","Kaul R","Pease C","Twomey PJ"],"additional_accession":[]},"is_claimable":false,"name":"Interlaboratory comparison of 25-hydroxyvitamin D assays: Vitamin D Standardization Program (VDSP) Intercomparison Study 2 - Part 2 ligand binding assays - impact of 25-hydroxyvitamin D<sub>2</sub> and 24R,25-dihydroxyvitamin D<sub>3</sub> on assay performance.","description":"An interlaboratory comparison study was conducted by the Vitamin D Standardization Program (VDSP) to assess the performance of ligand binding assays (Part 2) for the determination of serum total 25-hydroxyvitamin D [25(OH)D]. Fifty single-donor samples were assigned target values for concentrations of 25-hydroxyvitamin D<sub>2</sub> [25(OH)D<sub>2</sub>], 25-hydroxyvitamin D<sub>3</sub> [25(OH)D<sub>3</sub>], 3-epi-25-hydroxyvitamin D<sub>3</sub> [3-epi-25(OH)D<sub>3</sub>], and 24R,25-dihydroxyvitamin D<sub>3</sub> [24R,25(OH)<sub>2</sub>D<sub>3</sub>] using isotope dilution liquid chromatography-tandem mass spectrometry (ID LC-MS/MS). VDSP Intercomparison Study 2 Part 2 includes results from 17 laboratories using 32 ligand binding assays. Assay performance was evaluated using mean % bias","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Jan","modification":"2025-04-19T18:08:20.699Z","creation":"2025-04-19T18:08:20.699Z"},"accession":"S-EPMC8748290","cross_references":{"pubmed":["34435207"],"doi":["10.1007/s00216-021-03577-0"]}}