biostudies-literatureUnknown29Lan RGrowth differentiation factor 5 (<i>Gdf5</i>) and doublecortin (<i>Dcx</i>) genes are both expressed in joint interzone cells during synovial joint development. In this study, we re-analyzed the single cell RNA-sequencing data (Gene Expression Omnibus GSE151985) generated from <i>Gdf5</i> ⁺ cells of mouse knee joints at embryonic stages of E12.5, E13.5, E14.5, and E15.5, with a new focus on <i>Dcx</i>. We found that <i>Dcx</i> expression was enriched in clusters of <i>Gdf5</i> ⁺ cells, with high expression levels of pro-chondrogenic genes including sex determining region Y-box transcription factor 5 (<i>Sox5</i>), <i>Sox6</i>, <i>Sox9</i>, <i>Gdf5</i>, versican, matrilin 4, collagen type II α 1 chain (<i>Col2a1</i>), <i>Col9a1</i>, <i>Col9a2</i>, and <i>Col9a3</i> at Biochemistry and biophysics reports101200https://www.ebi.ac.uk/biostudies/studies/S-EPMC8749014biostudies-literature<i>Dcx</i> expression defines a subpopulation of <i>Gdf5</i> ⁺ cells with chondrogenic potentials in E12.5 mouse embryonic limbs.PMC8749014Ge DYou ZLiu YZLan Rfalse<i>Dcx</i> expression defines a subpopulation of <i>Gdf5</i> ⁺ cells with chondrogenic potentials in E12.5 mouse embryonic limbs.Growth differentiation factor 5 (<i>Gdf5</i>) and doublecortin (<i>Dcx</i>) genes are both expressed in joint interzone cells during synovial joint development. In this study, we re-analyzed the single cell RNA-sequencing data (Gene Expression Omnibus GSE151985) generated from <i>Gdf5</i> ⁺ cells of mouse knee joints at embryonic stages of E12.5, E13.5, E14.5, and E15.5, with a new focus on <i>Dcx</i>. We found that <i>Dcx</i> expression was enriched in clusters of <i>Gdf5</i> ⁺ cells, with high expression levels of pro-chondrogenic genes including sex determining region Y-box transcription factor 5 (<i>Sox5</i>), <i>Sox6</i>, <i>Sox9</i>, <i>Gdf5</i>, versican, matrilin 4, collagen type II α 1 chain (<i>Col2a1</i>), <i>Col9a1</i>, <i>Col9a2</i>, and <i>Col9a3</i> at 2022-01-01T00:00:00Z2022 Mar2026-05-09T05:55:51.534Z2022-02-11T16:20:28.056ZS-EPMC87490143503658610.1016/j.bbrep.2022.101200