biostudies-literatureUnknown10(12)Shen C<h4>Background</h4>A major goal of spermiation is to degrade the apical ectoplasmic specialization (ES) junction between Sertoli cells and elongating spermatids in preparation for the eventual disengagement of spermatids into the lumen. E3 ubiquitin ligases mediate the process of ubiquitination and the subsequent proteasomal degradation, but their specific role during spermiation remains largely unexplored.<h4>Methods</h4>Ankyrin repeat and SOCS box protein 17 (<i>Asb17</i>)-knockout mice were generated via a CRISPR/Cas9 approach. Epididymal sperm parameters were assessed by a computer-assisted sperm analysis (CASA) system, and morphological analysis of testicular tissues were performed based on histological and immunostaining staining, and transmission electron microscopy (TEM). The interTranslational andrology and urology4320-4332https://www.ebi.ac.uk/biostudies/studies/S-EPMC8749070biostudies-literatureE3 ubiquitin ligase ASB17 is required for spermiation in mice.PMC8749070Lv JZhou QZhang XHuang XZheng BShen CChen XYu JXu JLin MWu YfalseE3 ubiquitin ligase ASB17 is required for spermiation in mice.<h4>Background</h4>A major goal of spermiation is to degrade the apical ectoplasmic specialization (ES) junction between Sertoli cells and elongating spermatids in preparation for the eventual disengagement of spermatids into the lumen. E3 ubiquitin ligases mediate the process of ubiquitination and the subsequent proteasomal degradation, but their specific role during spermiation remains largely unexplored.<h4>Methods</h4>Ankyrin repeat and SOCS box protein 17 (<i>Asb17</i>)-knockout mice were generated via a CRISPR/Cas9 approach. Epididymal sperm parameters were assessed by a computer-assisted sperm analysis (CASA) system, and morphological analysis of testicular tissues were performed based on histological and immunostaining staining, and transmission electron microscopy (TEM). The inter2021-01-01T00:00:00Z2021 Dec2025-04-04T19:40:36.037Z2025-04-04T19:40:36.037ZS-EPMC87490703507081410.21037/tau-21-789