{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Lavelle K"],"funding":["Science Foundation Ireland","Science Foundation Ireland (SFI)"],"pagination":["e0172321"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8752142"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["88(1)"],"pubmed_abstract":["Four <u>b</u>acteriophage-<u>i</u>nsensitive <u>m</u>utants (BIMs) of the dairy starter bacterium Streptococcus thermophilus UCCSt50 were isolated following challenge with Brussowvirus SW13. The BIMs displayed an altered sedimentation phenotype. Whole-genome sequencing and comparative genomic analysis of the BIMs uncovered mutations within a family 2 glycosyltransferase-encoding gene (<i>orf</i>06955<sub>UCCSt50</sub>) located within the variable region of the cell wall-associated <u>r</u>hamnose-<u>g</u>lucose <u>p</u>olymer (Rgp) biosynthesis locus (designated the <i>rgp</i> gene cluster here). Complementation of a representative BIM, S. thermophilus B1, with native <i>orf</i>06955<sub>UCCSt50</sub> restored phage sensitivity comparable to that of the parent strain. Detailed bioinformati"],"journal":["Applied and environmental microbiology"],"pubmed_title":["Brussowvirus SW13 Requires a Cell Surface-Associated Polysaccharide To Recognize Its Streptococcus thermophilus Host."],"pmcid":["PMC8752142"],"funding_grant_id":["SFI/12/RC/2273-PI","15/SIRG/3430","13/1A/1953","SFI/12/RC/2273-P2"],"pubmed_authors":["Sadovskaya I","Ventura M","Lugli GA","Lavelle K","Kelleher P","Vinogradov E","Mahony J","van Sinderen D"],"additional_accession":[]},"is_claimable":false,"name":"Brussowvirus SW13 Requires a Cell Surface-Associated Polysaccharide To Recognize Its Streptococcus thermophilus Host.","description":"Four <u>b</u>acteriophage-<u>i</u>nsensitive <u>m</u>utants (BIMs) of the dairy starter bacterium Streptococcus thermophilus UCCSt50 were isolated following challenge with Brussowvirus SW13. The BIMs displayed an altered sedimentation phenotype. Whole-genome sequencing and comparative genomic analysis of the BIMs uncovered mutations within a family 2 glycosyltransferase-encoding gene (<i>orf</i>06955<sub>UCCSt50</sub>) located within the variable region of the cell wall-associated <u>r</u>hamnose-<u>g</u>lucose <u>p</u>olymer (Rgp) biosynthesis locus (designated the <i>rgp</i> gene cluster here). Complementation of a representative BIM, S. thermophilus B1, with native <i>orf</i>06955<sub>UCCSt50</sub> restored phage sensitivity comparable to that of the parent strain. Detailed bioinformati","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Jan","modification":"2026-06-12T08:50:51.808Z","creation":"2026-06-12T03:08:33.451Z"},"accession":"S-EPMC8752142","cross_references":{"pubmed":["34669424"],"doi":["10.1128/AEM.01723-21"]}}