biostudies-literatureLavelle KScience Foundation IrelandScience Foundation Ireland (SFI)e0172321https://www.ebi.ac.uk/biostudies/studies/S-EPMC8752142biostudies-literatureUnknown88(1)Four <u>b</u>acteriophage-<u>i</u>nsensitive <u>m</u>utants (BIMs) of the dairy starter bacterium Streptococcus thermophilus UCCSt50 were isolated following challenge with Brussowvirus SW13. The BIMs displayed an altered sedimentation phenotype. Whole-genome sequencing and comparative genomic analysis of the BIMs uncovered mutations within a family 2 glycosyltransferase-encoding gene (<i>orf</i>06955_UCCSt50) located within the variable region of the cell wall-associated <u>r</u>hamnose-<u>g</u>lucose <u>p</u>olymer (Rgp) biosynthesis locus (designated the <i>rgp</i> gene cluster here). Complementation of a representative BIM, S. thermophilus B1, with native <i>orf</i>06955_UCCSt50 restored phage sensitivity comparable to that of the parent strain. Detailed bioinformatiApplied and environmental microbiologyBrussowvirus SW13 Requires a Cell Surface-Associated Polysaccharide To Recognize Its Streptococcus thermophilus Host.PMC8752142SFI/12/RC/2273-PI15/SIRG/343013/1A/1953SFI/12/RC/2273-P2Sadovskaya IVentura MLugli GALavelle KKelleher PVinogradov EMahony Jvan Sinderen DfalseBrussowvirus SW13 Requires a Cell Surface-Associated Polysaccharide To Recognize Its Streptococcus thermophilus Host.Four <u>b</u>acteriophage-<u>i</u>nsensitive <u>m</u>utants (BIMs) of the dairy starter bacterium Streptococcus thermophilus UCCSt50 were isolated following challenge with Brussowvirus SW13. The BIMs displayed an altered sedimentation phenotype. Whole-genome sequencing and comparative genomic analysis of the BIMs uncovered mutations within a family 2 glycosyltransferase-encoding gene (<i>orf</i>06955_UCCSt50) located within the variable region of the cell wall-associated <u>r</u>hamnose-<u>g</u>lucose <u>p</u>olymer (Rgp) biosynthesis locus (designated the <i>rgp</i> gene cluster here). Complementation of a representative BIM, S. thermophilus B1, with native <i>orf</i>06955_UCCSt50 restored phage sensitivity comparable to that of the parent strain. Detailed bioinformati2022-01-01T00:00:00Z2022 Jan2026-06-12T08:50:51.808Z2026-06-12T03:08:33.451ZS-EPMC87521423466942410.1128/AEM.01723-21