biostudies-literatureUnknown9(24)Ouyang L<h4>Background</h4>Pancreatic cancer (PC) can be considered a representative cancer type of the human body. As demonstrated by some studies, microRNA (miR)-499 is dysregulated in various cancer types including PC, for which chemotherapy involving 5-fluorouracil (5-FU) has long been considered the first-line therapy. However, there are complex and comprehensive mechanisms related to 5-FU, which have not been fully elucidated. This study thus aimed to examine the molecular mechanisms of 5-FU resistance through miR-499a-5p in PC.<h4>Methods</h4>The expression of miR-499a-5p in PC was measured using quantitative polymerase chain reaction (PCR). MiR-499a-5p was examined <i>in-vivo</i> for its effects on the malignant phenotypes of PC cells.<h4>Results</h4>The results of the present study demonsAnnals of translational medicine1798https://www.ebi.ac.uk/biostudies/studies/S-EPMC8756217biostudies-literatureMiR-499a-5p promotes 5-FU resistance and the cell proliferation and migration through activating PI3K/Akt signaling by targeting PTEN in pancreatic cancer.PMC8756217Shang QCLei DQJin GZheng HLiu RDOuyang LLi HFHu XGfalseMiR-499a-5p promotes 5-FU resistance and the cell proliferation and migration through activating PI3K/Akt signaling by targeting PTEN in pancreatic cancer.<h4>Background</h4>Pancreatic cancer (PC) can be considered a representative cancer type of the human body. As demonstrated by some studies, microRNA (miR)-499 is dysregulated in various cancer types including PC, for which chemotherapy involving 5-fluorouracil (5-FU) has long been considered the first-line therapy. However, there are complex and comprehensive mechanisms related to 5-FU, which have not been fully elucidated. This study thus aimed to examine the molecular mechanisms of 5-FU resistance through miR-499a-5p in PC.<h4>Methods</h4>The expression of miR-499a-5p in PC was measured using quantitative polymerase chain reaction (PCR). MiR-499a-5p was examined <i>in-vivo</i> for its effects on the malignant phenotypes of PC cells.<h4>Results</h4>The results of the present study demons2021-01-01T00:00:00Z2021 Dec2025-04-20T00:39:41.666Z2025-02-19T03:29:20.583ZS-EPMC87562173507149210.21037/atm-21-6556