biostudies-literatureRogers-DeCotes AWNIDCR NIH HHSNHLBI NIH HHSAmerican Heart Association IncNational Institutes of Health547-557https://www.ebi.ac.uk/biostudies/studies/S-EPMC8759092biostudies-literatureUnknown29(4)<h4>Objective</h4>Determine the role of the extracellular matrix protease ADAMTS5 in development of the trabeculated bone of the mandibular condyle.<h4>Methods</h4>The mandibular condyles of wild type and mice deficient in the protease ADAMTS5 were examined for histopathology with Safranin O staining. Microcomputed tomography was performed to analyze the developing bone of the mandibular condyle. RNAscope and immunohistochemistry were utilized to investigate cell type and extracellular matrix expression.<h4>Results</h4>Mice deficient in Adamts5, (Adamts5^tm1Dgen/J) exhibit an increase in trabecular separation (n = 37 wild type; n = 27: P < 0.0001) and reduction of trabecular thickness P = 0.0116 and bone volume fraction P = 0.0869 in the mandibular condylar head compared to wild Osteoarthritis and cartilageADAMTS5 is required for normal trabeculated bone development in the mandibular condyle.PMC8759092R01 HL121382F30 DE028180T32 DE017551Rogers-DeCotes AWKern CBPorto SCDupuis LEfalseADAMTS5 is required for normal trabeculated bone development in the mandibular condyle.<h4>Objective</h4>Determine the role of the extracellular matrix protease ADAMTS5 in development of the trabeculated bone of the mandibular condyle.<h4>Methods</h4>The mandibular condyles of wild type and mice deficient in the protease ADAMTS5 were examined for histopathology with Safranin O staining. Microcomputed tomography was performed to analyze the developing bone of the mandibular condyle. RNAscope and immunohistochemistry were utilized to investigate cell type and extracellular matrix expression.<h4>Results</h4>Mice deficient in Adamts5, (Adamts5^tm1Dgen/J) exhibit an increase in trabecular separation (n = 37 wild type; n = 27: P < 0.0001) and reduction of trabecular thickness P = 0.0116 and bone volume fraction P = 0.0869 in the mandibular condylar head compared to wild 2021-01-01T00:00:00Z2021 Apr2025-04-22T09:18:08.288Z2025-04-05T22:58:17.498ZS-EPMC87590923356154010.1016/j.joca.2021.01.005