biostudies-literatureLiu ZChina National Funds for Distinguished Young ScientistsShanghai Science and Technology Committee ProgramNational Natural Science Foundation of Chinaj.issn.2095-3941.2020.0343https://www.ebi.ac.uk/biostudies/studies/S-EPMC8763008biostudies-literatureUnknown19(1)<h4>Objective</h4>The dysregulation of ribosome biogenesis is associated with the progression of numerous tumors, including hepatocellular carcinoma (HCC). Small nucleolar RNAs (snoRNAs) regulate ribosome biogenesis by guiding the modification of ribosomal RNAs (rRNAs). However, the underlying mechanism of this process in HCC remains elusive.<h4>Methods</h4>RNA immunoprecipitation and sequencing were used to analyze RNAs targeted by ribosome proteins. The biological functions of SNORA23 were examined in HCC cells and a xenograft mouse model. To elucidate the underlying mechanisms, the 2'-O-ribose methylation level of rRNAs was evaluated by qPCR, and the key proteins in the PI3K/Akt/mTOR pathway were detected using Western blot.<h4>Results</h4>Twelve snoRNAs were found to co-exist in 4 cancCancer biology & medicineSNORA23 inhibits HCC tumorigenesis by impairing the 2'-O-ribose methylation level of 28S rRNA.PMC8763008814250193200178618XD1405300Li WLiu HQin QLiu ZJing JWang RJia YLiu SPang YfalseSNORA23 inhibits HCC tumorigenesis by impairing the 2'-O-ribose methylation level of 28S rRNA.<h4>Objective</h4>The dysregulation of ribosome biogenesis is associated with the progression of numerous tumors, including hepatocellular carcinoma (HCC). Small nucleolar RNAs (snoRNAs) regulate ribosome biogenesis by guiding the modification of ribosomal RNAs (rRNAs). However, the underlying mechanism of this process in HCC remains elusive.<h4>Methods</h4>RNA immunoprecipitation and sequencing were used to analyze RNAs targeted by ribosome proteins. The biological functions of SNORA23 were examined in HCC cells and a xenograft mouse model. To elucidate the underlying mechanisms, the 2'-O-ribose methylation level of rRNAs was evaluated by qPCR, and the key proteins in the PI3K/Akt/mTOR pathway were detected using Western blot.<h4>Results</h4>Twelve snoRNAs were found to co-exist in 4 canc2021-01-01T00:00:00Z2021 Mar2026-05-31T09:47:10.549Z2025-04-21T17:10:25.319ZS-EPMC87630083371080410.20892/j.issn.2095-3941.2020.0343