{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Lo T"],"funding":["National Center for Advancing Translational Sciences","NCATS NIH HHS","NHLBI NIH HHS","National Heart, Lung, and Blood Institute","NIH","Harvard Catalyst","U.S. National Institutes of Health","NIH HHS"],"pagination":["e619-e630"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8764221"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["107(2)"],"pubmed_abstract":["<h4>Objective</h4>To characterize longitudinal changes in blood biomarkers, leukocyte composition, and gene expression following laparoscopic sleeve gastrectomy (LSG).<h4>Background</h4>LSG is an effective treatment for obesity, leading to sustainable weight loss and improvements in obesity-related comorbidities and inflammatory profiles. However, the effects of LSG on immune function and metabolism remain uncertain.<h4>Methods</h4>Prospective data were collected from 23 enrolled human subjects from a single institution. Parameters of weight, comorbidities, and trends in blood biomarkers and leukocyte subsets were observed from preoperative baseline to 1 year postsurgery in 3-month follow-up intervals. RNA sequencing was performed on pairs of whole blood samples from the first 6 subjects of the study (baseline and 3 months postsurgery) to identify genome-wide gene expression changes associated with undergoing LSG.<h4>Results</h4>LSG led to a significant decrease in mean total body weight loss (18.1%) at 3 months and among diabetic subjects a reduction in hemoglobin A1c. Improvements in clinical inflammatory and hormonal biomarkers were demonstrated as early as 3 months after LSG. A reduction in neutrophil-lymphocyte ratio was observed, driven by a reduction in absolute neutrophil counts. Gene set enrichment analyses of differential whole blood gene expression demonstrated that after 3 months LSG induced transcriptomic changes not only in inflammatory cytokine pathways but also in several key metabolic pathways related to energy metabolism.<h4>Conclusions</h4>LSG induces significant changes in the composition and metabolism of immune cells as early as 3 months postoperatively. Further evaluation is required of bariatric surgery's effects on immunometabolism and the consequences for host defense and metabolic disease."],"journal":["The Journal of clinical endocrinology and metabolism"],"pubmed_title":["Early Changes in Immune Cell Count, Metabolism, and Function Following Sleeve Gastrectomy: A Prospective Human Study."],"pmcid":["PMC8764221"],"funding_grant_id":["R01 HL086601","KL2 TR002542","UL 1TR002541","K01 HL127265"],"pubmed_authors":["Haridas RS","Rudge EJM","Heshmati K","Croteau-Chonka DC","Weigl AM","McNutt SW","Ituah CO","Iyoha-Bello OJ","Tavakkoli A","Chase RP","Sathe L","Lucey EM","Lo T","Raby BA","Farnam L","Benjamin EJ","Sheu EG"],"additional_accession":[]},"is_claimable":false,"name":"Early Changes in Immune Cell Count, Metabolism, and Function Following Sleeve Gastrectomy: A Prospective Human Study.","description":"<h4>Objective</h4>To characterize longitudinal changes in blood biomarkers, leukocyte composition, and gene expression following laparoscopic sleeve gastrectomy (LSG).<h4>Background</h4>LSG is an effective treatment for obesity, leading to sustainable weight loss and improvements in obesity-related comorbidities and inflammatory profiles. However, the effects of LSG on immune function and metabolism remain uncertain.<h4>Methods</h4>Prospective data were collected from 23 enrolled human subjects from a single institution. Parameters of weight, comorbidities, and trends in blood biomarkers and leukocyte subsets were observed from preoperative baseline to 1 year postsurgery in 3-month follow-up intervals. RNA sequencing was performed on pairs of whole blood samples from the first 6 subjects of the study (baseline and 3 months postsurgery) to identify genome-wide gene expression changes associated with undergoing LSG.<h4>Results</h4>LSG led to a significant decrease in mean total body weight loss (18.1%) at 3 months and among diabetic subjects a reduction in hemoglobin A1c. Improvements in clinical inflammatory and hormonal biomarkers were demonstrated as early as 3 months after LSG. A reduction in neutrophil-lymphocyte ratio was observed, driven by a reduction in absolute neutrophil counts. Gene set enrichment analyses of differential whole blood gene expression demonstrated that after 3 months LSG induced transcriptomic changes not only in inflammatory cytokine pathways but also in several key metabolic pathways related to energy metabolism.<h4>Conclusions</h4>LSG induces significant changes in the composition and metabolism of immune cells as early as 3 months postoperatively. Further evaluation is required of bariatric surgery's effects on immunometabolism and the consequences for host defense and metabolic disease.","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Jan","modification":"2024-11-21T01:37:42.909Z","creation":"2024-11-21T01:37:42.909Z"},"accession":"S-EPMC8764221","cross_references":{"pubmed":["34514501"],"doi":["10.1210/clinem/dgab673"]}}