{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Li YP"],"funding":["National Natural Science Foundation of China"],"pagination":["e0010090"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8765647"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["16(1)"],"pubmed_abstract":["<h4>Background</h4>This research aimed to explore the association between the RIG-I-like receptor (RIG-I and MDA5 encoded by DDX58 and IFIH1, respectively) pathways and the risk or severity of hand, foot, and mouth disease caused by enterovirus 71 (EV71-HFMD). In this context, we explored the influence of gene methylation and polymorphism on EV71-HFMD.<h4>Methodology/principal findings</h4>60 healthy controls and 120 EV71-HFMD patients, including 60 mild EV71-HFMD and 60 severe EV71-HFMD patients, were enrolled. First, MiSeq was performed to explore the methylation of CpG islands in the DDX58 and IFIH1 promoter regions. Then, DDX58 and IFIH1 expression were detected in PBMCs using RT-qPCR. Finally, imLDR was used to detect DDX58 and IFIH1 single-nucleotide polymorphism (SNP) genotypes. Sev"],"journal":["PLoS neglected tropical diseases"],"pubmed_title":["DNA methylation and single-nucleotide polymorphisms in DDX58 are associated with hand, foot and mouth disease caused by enterovirus 71."],"pmcid":["PMC8765647"],"funding_grant_id":["81701632"],"pubmed_authors":["Wang MQ","Dang SS","Zhai S","Deng HL","Zhang YF","Li YP","Tian Y","Liu CR","Chen Y"],"additional_accession":[]},"is_claimable":false,"name":"DNA methylation and single-nucleotide polymorphisms in DDX58 are associated with hand, foot and mouth disease caused by enterovirus 71.","description":"<h4>Background</h4>This research aimed to explore the association between the RIG-I-like receptor (RIG-I and MDA5 encoded by DDX58 and IFIH1, respectively) pathways and the risk or severity of hand, foot, and mouth disease caused by enterovirus 71 (EV71-HFMD). In this context, we explored the influence of gene methylation and polymorphism on EV71-HFMD.<h4>Methodology/principal findings</h4>60 healthy controls and 120 EV71-HFMD patients, including 60 mild EV71-HFMD and 60 severe EV71-HFMD patients, were enrolled. First, MiSeq was performed to explore the methylation of CpG islands in the DDX58 and IFIH1 promoter regions. Then, DDX58 and IFIH1 expression were detected in PBMCs using RT-qPCR. Finally, imLDR was used to detect DDX58 and IFIH1 single-nucleotide polymorphism (SNP) genotypes. Sev","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Jan","modification":"2026-05-28T23:34:14.927Z","creation":"2022-02-11T16:14:57.79Z"},"accession":"S-EPMC8765647","cross_references":{"pubmed":["35041675"],"doi":["10.1371/journal.pntd.0010090"]}}