biostudies-literatureUnknown9Tong FChiral amino alcohols are prevalent synthons in pharmaceuticals and synthetic bioactive compounds. The efficient synthesis of chiral amino alcohols using ammonia as the sole amino donor under mild conditions is highly desired and challenging in organic chemistry and biotechnology. Our previous work explored a panel of engineered amine dehydrogenases (AmDHs) derived from amino acid dehydrogenase (AADH), enabling the one-step synthesis of chiral amino alcohols via the asymmetric reductive amination of α-hydroxy ketones. Although the AmDH-directed asymmetric reduction is in a high stereoselective manner, the activity is yet fully excavated. Herein, an engineered AmDH derived from a leucine dehydrogenase from <i>Sporosarcina psychrophila</i> (<i>Sp</i>AmDH) was recruited as the starting enzymeFrontiers in bioengineering and biotechnology778584https://www.ebi.ac.uk/biostudies/studies/S-EPMC8766677biostudies-literatureBiosynthesis of Chiral Amino Alcohols via an Engineered Amine Dehydrogenase in <i>E. coli</i>.PMC8766677Jiang YLi JQin ZXiao YWang HTong FMing HQu GSun ZfalseBiosynthesis of Chiral Amino Alcohols via an Engineered Amine Dehydrogenase in <i>E. coli</i>.Chiral amino alcohols are prevalent synthons in pharmaceuticals and synthetic bioactive compounds. The efficient synthesis of chiral amino alcohols using ammonia as the sole amino donor under mild conditions is highly desired and challenging in organic chemistry and biotechnology. Our previous work explored a panel of engineered amine dehydrogenases (AmDHs) derived from amino acid dehydrogenase (AADH), enabling the one-step synthesis of chiral amino alcohols via the asymmetric reductive amination of α-hydroxy ketones. Although the AmDH-directed asymmetric reduction is in a high stereoselective manner, the activity is yet fully excavated. Herein, an engineered AmDH derived from a leucine dehydrogenase from <i>Sporosarcina psychrophila</i> (<i>Sp</i>AmDH) was recruited as the starting enzyme2021-01-01T00:00:00Z20212026-07-14T14:01:21.217Z2026-06-21T03:06:23.17ZS-EPMC87666773507120010.3389/fbioe.2021.778584