{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Krasemann S"],"funding":["Bundesministerium für Bildung und Forschung","Deutsche Forschungsgemeinschaft","Fraunhofer-Gesellschaft","BMBF Berlin","icare"],"pagination":["307-320"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8772030"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["17(2)"],"pubmed_abstract":["Neurological complications are common in COVID-19. Although SARS-CoV-2 has been detected in patients' brain tissues, its entry routes and resulting consequences are not well understood. Here, we show a pronounced upregulation of interferon signaling pathways of the neurovascular unit in fatal COVID-19. By investigating the susceptibility of human induced pluripotent stem cell (hiPSC)-derived brain capillary endothelial-like cells (BCECs) to SARS-CoV-2 infection, we found that BCECs were infected and recapitulated transcriptional changes detected in vivo. While BCECs were not compromised in their paracellular tightness, we found SARS-CoV-2 in the basolateral compartment in transwell assays after apical infection, suggesting active replication and transcellular transport of virus across the "],"journal":["Stem cell reports"],"pubmed_title":["The blood-brain barrier is dysregulated in COVID-19 and serves as a CNS entry route for SARS-CoV-2."],"pmcid":["PMC8772030"],"funding_grant_id":["840260","FR1720/18-1","01EK1608B","602015","GL589/10-1","01EK1608A","01KX2021"],"pubmed_authors":["Cubukova A","Zaliani A","Franzenburg S","Liang Y","Moese S","Matschke J","Franke A","Claussen C","Zhang L","Littau JL","Ton K","Sepulveda-Falla D","Gribbon P","Gerhartl A","Thies E","Glatzel M","Ricklefs F","Pless O","Bodem J","Friese MA","Brachner A","Haferkamp U","Muller FJ","Hartmann K","Neuhaus W","Kannt A","Geisslinger G","Geiger N","Pfefferle S","Fitzek A","Woo MS","Heinrich F","Appelt-Menzel A","Konig EM","Leu J","Krasemann S","Barenberg J","Sperhake J","Ondruschka B","Sauvigny T","Schweizer M"],"additional_accession":[]},"is_claimable":false,"name":"The blood-brain barrier is dysregulated in COVID-19 and serves as a CNS entry route for SARS-CoV-2.","description":"Neurological complications are common in COVID-19. Although SARS-CoV-2 has been detected in patients' brain tissues, its entry routes and resulting consequences are not well understood. Here, we show a pronounced upregulation of interferon signaling pathways of the neurovascular unit in fatal COVID-19. By investigating the susceptibility of human induced pluripotent stem cell (hiPSC)-derived brain capillary endothelial-like cells (BCECs) to SARS-CoV-2 infection, we found that BCECs were infected and recapitulated transcriptional changes detected in vivo. While BCECs were not compromised in their paracellular tightness, we found SARS-CoV-2 in the basolateral compartment in transwell assays after apical infection, suggesting active replication and transcellular transport of virus across the ","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Feb","modification":"2026-05-09T20:36:16.88Z","creation":"2022-02-11T16:02:44.029Z"},"accession":"S-EPMC8772030","cross_references":{"pubmed":["35063125"],"doi":["10.1016/j.stemcr.2021.12.011"]}}