biostudies-literatureSamak MDeutsche SonderforschungsbereichEuropean Research Council58https://www.ebi.ac.uk/biostudies/studies/S-EPMC8773250biostudies-literatureUnknown10(1)Microvascular dysfunction is a pathological hallmark of diabetes, and is central to the ethology of diabetes-associated cardiac events. Herein, previous studies have highlighted the role of the vasoactive micro-RNA 92a (miR-92a) in small, as well as large, animal models. In this study, we explore the effects of miR-92a on mouse and human cardiac microvascular endothelial cells (MCMEC, HCMEC), and its underlying molecular mechanisms. Diabetic HCMEC displayed impaired angiogenesis and a pronounced inflammatory phenotype. Quantitative PCR (qPCR) showed an upregulation of miR-92a in primary diabetic HCMEC. Downregulation of miR-92a by antagomir transfection in diabetic HCMEC rescued angiogenesis and ameliorated diabetic endothelial bed inflammation. Furthermore, additional analysis of potentiaBiomedicinesMicro-RNA 92a as a Therapeutic Target for Cardiac Microvascular Dysfunction in Diabetes.PMC8773250CRC127ERC StG 758087758087Kues ALe Noble FKaltenborn DHinkel RGermena GSamak MfalseMicro-RNA 92a as a Therapeutic Target for Cardiac Microvascular Dysfunction in Diabetes.Microvascular dysfunction is a pathological hallmark of diabetes, and is central to the ethology of diabetes-associated cardiac events. Herein, previous studies have highlighted the role of the vasoactive micro-RNA 92a (miR-92a) in small, as well as large, animal models. In this study, we explore the effects of miR-92a on mouse and human cardiac microvascular endothelial cells (MCMEC, HCMEC), and its underlying molecular mechanisms. Diabetic HCMEC displayed impaired angiogenesis and a pronounced inflammatory phenotype. Quantitative PCR (qPCR) showed an upregulation of miR-92a in primary diabetic HCMEC. Downregulation of miR-92a by antagomir transfection in diabetic HCMEC rescued angiogenesis and ameliorated diabetic endothelial bed inflammation. Furthermore, additional analysis of potentia2021-01-01T00:00:00Z2021 Dec2026-06-19T03:09:53.895Z2022-02-11T16:04:54.476ZS-EPMC87732503505273810.3390/biomedicines10010058