{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Munoz-Garcia N"],"funding":["INTERREG POCTEP Spain-Portugal","Fondo Europeo de Desarrollo Regional","Instituto de Salud Carlos III","Centro de Investigación Biomédica en Red de Cáncer"],"pagination":["408"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8773687"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["14(2)"],"pubmed_abstract":["Flow cytometric (FCM) analysis of the constant region 1 of the T-cell receptor β chain (TRBC1) expression for assessing Tαβ-cell clonality has been recently validated. However, its utility for the diagnosis of clonality of T-large granular lymphocytic leukemia (T-LGLL) needs to be confirmed, since more mature Tαβ cells (i.e., T-LGL normal-counterpart) show broader TRBC1+/TRBC1- ratios vs. total Tαβ cells. We compared the distribution and absolute counts of TRBC1+ and TRBC1- Tαβ-LGL in blood containing polyclonal (n = 25) vs. clonal (n = 29) LGL. Overall, polyclonal TRBC1+ or TRBC1- Tαβ-LGL ranged between 0.36 and 571 cells/μL (3.2-91% TRBC1+ cells), whereas the clonal LGL cases showed between 51 and 11,678 cells/μL (<0.9% or >96% TRBC1+ cells). Among the distinct TCRVβ families, the CD28- effector-memory and terminal-effector polyclonal Tαβ cells ranged between 0 and 25 TRBC1+ or TRBC1- cells/μL and between 0 and 100% TRBC1+ cells, while clonal LGL ranged between 32 and 5515 TRBC1+ or TRBC1- cells/μL, representing <1.6% or >98% TRBC1+ cells. Our data support the utility of the TRBC1-FCM assay for detecting T-cell clonality in expansions of Tαβ-LGL suspected of T-LGLL based on altered percentages of TRBC1+ Tαβ cells. However, in the absence of lymphocytosis or in the case of TαβCD4-LGL expansion, the detection of increased absolute cell counts by the TRBC1-FCM assay for more accurately defined subpopulations of Tαβ-LGL-expressing individual TCRVβ families, allows the detection of T-cell clonality, even in the absence of phenotypic aberrations."],"journal":["Cancers"],"pubmed_title":["High-Sensitive TRBC1-Based Flow Cytometric Assessment of T-Cell Clonality in Tαβ-Large Granular Lymphocytic Leukemia."],"pmcid":["PMC8773687"],"funding_grant_id":["0639-IDIAL-NET-3-3","CB16/12/00400","EuroFlow","PI20-01346","IBPredoc17/00012"],"pubmed_authors":["Mateos S","Moran-Plata FJ","van Dongen JJM","Lima M","Barrena S","Munoz-Garcia N","Villamor N","Almeida J","Orfao A","Caldas C"],"additional_accession":[]},"is_claimable":false,"name":"High-Sensitive TRBC1-Based Flow Cytometric Assessment of T-Cell Clonality in Tαβ-Large Granular Lymphocytic Leukemia.","description":"Flow cytometric (FCM) analysis of the constant region 1 of the T-cell receptor β chain (TRBC1) expression for assessing Tαβ-cell clonality has been recently validated. However, its utility for the diagnosis of clonality of T-large granular lymphocytic leukemia (T-LGLL) needs to be confirmed, since more mature Tαβ cells (i.e., T-LGL normal-counterpart) show broader TRBC1+/TRBC1- ratios vs. total Tαβ cells. We compared the distribution and absolute counts of TRBC1+ and TRBC1- Tαβ-LGL in blood containing polyclonal (n = 25) vs. clonal (n = 29) LGL. Overall, polyclonal TRBC1+ or TRBC1- Tαβ-LGL ranged between 0.36 and 571 cells/μL (3.2-91% TRBC1+ cells), whereas the clonal LGL cases showed between 51 and 11,678 cells/μL (<0.9% or >96% TRBC1+ cells). Among the distinct TCRVβ families, the CD28- effector-memory and terminal-effector polyclonal Tαβ cells ranged between 0 and 25 TRBC1+ or TRBC1- cells/μL and between 0 and 100% TRBC1+ cells, while clonal LGL ranged between 32 and 5515 TRBC1+ or TRBC1- cells/μL, representing <1.6% or >98% TRBC1+ cells. Our data support the utility of the TRBC1-FCM assay for detecting T-cell clonality in expansions of Tαβ-LGL suspected of T-LGLL based on altered percentages of TRBC1+ Tαβ cells. However, in the absence of lymphocytosis or in the case of TαβCD4-LGL expansion, the detection of increased absolute cell counts by the TRBC1-FCM assay for more accurately defined subpopulations of Tαβ-LGL-expressing individual TCRVβ families, allows the detection of T-cell clonality, even in the absence of phenotypic aberrations.","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Jan","modification":"2024-02-15T07:27:10.983Z","creation":"2022-02-11T16:04:53.126Z"},"accession":"S-EPMC8773687","cross_references":{"pubmed":["35053571"],"doi":["10.3390/cancers14020408"]}}