{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Myerson JW"],"funding":["American Heart Association","United States Department of Defense | Defense Threat Reduction Agency","NIBIB NIH HHS","NCATS NIH HHS","NHLBI NIH HHS","U.S. Department of Health & Human Services | National Institutes of Health"],"pagination":["86-97"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8776575"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["17(1)"],"pubmed_abstract":["This study shows that the supramolecular arrangement of proteins in nanoparticle structures predicts nanoparticle accumulation in neutrophils in acute lung inflammation (ALI). We observed homing to inflamed lungs for a variety of nanoparticles with agglutinated protein (NAPs), defined by arrangement of protein in or on the nanoparticles via hydrophobic interactions, crosslinking and electrostatic interactions. Nanoparticles with symmetric protein arrangement (for example, viral capsids) had no selectivity for inflamed lungs. Flow cytometry and immunohistochemistry showed NAPs have tropism for pulmonary neutrophils. Protein-conjugated liposomes were engineered to recapitulate NAP tropism for pulmonary neutrophils. NAP uptake in neutrophils was shown to depend on complement opsonization. We demonstrate diagnostic imaging of ALI with NAPs; show NAP tropism for inflamed human donor lungs; and show that NAPs can remediate pulmonary oedema in ALI. This work demonstrates that structure-dependent tropism for neutrophils drives NAPs to inflamed lungs and shows NAPs can detect and treat ALI."],"journal":["Nature nanotechnology"],"pubmed_title":["Supramolecular arrangement of protein in nanoparticle structures predicts nanoparticle tropism for neutrophils in acute lung inflammation."],"pmcid":["PMC8776575"],"funding_grant_id":["T32 HL007915","R01 HL157189","R01 HL153510","R01 HL125462","K08 HL130430","F31 HL154662","EB022641","UL1 TR001878","F32 HL151026","K08 HL138269","R01HL125462","HDTRA-1-15-0045","R01 HL160694","19CDA345900001","T32HL07915","UL1TR001878","R01 EB022641"],"pubmed_authors":["Wu J","Myerson JW","Zhang HY","Grosser T","Mazaleuskaya LL","Rubey KM","Habibi N","Rotello VM","Gregory JV","Luther DC","Mitragotri S","Greineder CF","Marcos-Contreras OA","Lahann J","Worthen GS","Brenner JS","Zamora ME","Kiseleva RY","Glassman PM","Shuvaeva T","Ferguson LT","Nong J","Zaleski MH","Johnston I","Hood ED","Patel PN","Muzykantov VR","Lee YW","Walsh LR"],"additional_accession":[]},"is_claimable":false,"name":"Supramolecular arrangement of protein in nanoparticle structures predicts nanoparticle tropism for neutrophils in acute lung inflammation.","description":"This study shows that the supramolecular arrangement of proteins in nanoparticle structures predicts nanoparticle accumulation in neutrophils in acute lung inflammation (ALI). We observed homing to inflamed lungs for a variety of nanoparticles with agglutinated protein (NAPs), defined by arrangement of protein in or on the nanoparticles via hydrophobic interactions, crosslinking and electrostatic interactions. Nanoparticles with symmetric protein arrangement (for example, viral capsids) had no selectivity for inflamed lungs. Flow cytometry and immunohistochemistry showed NAPs have tropism for pulmonary neutrophils. Protein-conjugated liposomes were engineered to recapitulate NAP tropism for pulmonary neutrophils. NAP uptake in neutrophils was shown to depend on complement opsonization. We demonstrate diagnostic imaging of ALI with NAPs; show NAP tropism for inflamed human donor lungs; and show that NAPs can remediate pulmonary oedema in ALI. This work demonstrates that structure-dependent tropism for neutrophils drives NAPs to inflamed lungs and shows NAPs can detect and treat ALI.","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Jan","modification":"2025-04-18T13:24:54.224Z","creation":"2025-04-06T23:06:20.579Z"},"accession":"S-EPMC8776575","cross_references":{"pubmed":["34795440"],"doi":["10.1038/s41565-021-00997-y"]}}