{"database":"biostudies-literature","file_versions":[],"scores":{"citationCount":0,"reanalysisCount":0,"viewCount":60,"searchCount":0},"additional":{"omics_type":["Unknown"],"volume":["14(2)"],"submitter":["Jiao C"],"funding":["Deutsche Forschungsgemeinschaft"],"pubmed_abstract":["Stimuli-responsive hydrogels have a wide range of potential applications in microfluidics, which has drawn great attention. Double cross-linked hydrogels are very well suited for this application as they offer both stability and the required responsive behavior. Here, we report the integration of poly(<i>N</i>-isopropylacrylamide) (PNiPAAm) hydrogel with a permanent cross-linker (<i>N,N'</i>-methylenebisacrylamide, BIS) and a redox responsive reversible cross-linker (<i>N,N'</i>-bis(acryloyl)cystamine, BAC) into a microfluidic device through photopolymerization. Cleavage and re-formation of disulfide bonds introduced by BAC changed the cross-linking densities of the hydrogel dots, making them swell or shrink. Rheological measurements allowed for selecting hydrogels that withstand long-term shear forces present in microfluidic devices under continuous flow. Once implemented, the thiol-disulfide exchange allowed the hydrogel dots to successfully capture and release the protein bovine serum albumin (BSA). BSA was labeled with rhodamine B and functionalized with 2-(2-pyridyldithio)-ethylamine (PDA) to introduce disulfide bonds. The reversible capture and release of the protein reached an efficiency of 83.6% in release rate and could be repeated over 3 cycles within the microfluidic device. These results demonstrate that our redox-responsive hydrogel dots enable the dynamic capture and release of various different functionalized (macro)molecules (e.g., proteins and drugs) and have a great potential to be integrated into a lab-on-a-chip device for detection and/or delivery."],"journal":["Polymers"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8780672"],"repository":["biostudies-literature"],"pubmed_title":["Reversible Protein Capture and Release by Redox-Responsive Hydrogel in Microfluidics."],"pmcid":["PMC8780672"],"funding_grant_id":["GRK 1865"],"pubmed_authors":["Geisler M","Gaitzsch J","Obst F","Appelhans D","Voit B","Che Y","Jiao C","Richter A"],"view_count":["60"],"additional_accession":[]},"is_claimable":false,"name":"Reversible Protein Capture and Release by Redox-Responsive Hydrogel in Microfluidics.","description":"Stimuli-responsive hydrogels have a wide range of potential applications in microfluidics, which has drawn great attention. Double cross-linked hydrogels are very well suited for this application as they offer both stability and the required responsive behavior. Here, we report the integration of poly(<i>N</i>-isopropylacrylamide) (PNiPAAm) hydrogel with a permanent cross-linker (<i>N,N'</i>-methylenebisacrylamide, BIS) and a redox responsive reversible cross-linker (<i>N,N'</i>-bis(acryloyl)cystamine, BAC) into a microfluidic device through photopolymerization. Cleavage and re-formation of disulfide bonds introduced by BAC changed the cross-linking densities of the hydrogel dots, making them swell or shrink. Rheological measurements allowed for selecting hydrogels that withstand long-term shear forces present in microfluidic devices under continuous flow. Once implemented, the thiol-disulfide exchange allowed the hydrogel dots to successfully capture and release the protein bovine serum albumin (BSA). BSA was labeled with rhodamine B and functionalized with 2-(2-pyridyldithio)-ethylamine (PDA) to introduce disulfide bonds. The reversible capture and release of the protein reached an efficiency of 83.6% in release rate and could be repeated over 3 cycles within the microfluidic device. These results demonstrate that our redox-responsive hydrogel dots enable the dynamic capture and release of various different functionalized (macro)molecules (e.g., proteins and drugs) and have a great potential to be integrated into a lab-on-a-chip device for detection and/or delivery.","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Jan","modification":"2022-02-11T16:02:40.174Z","creation":"2022-02-11T16:02:40.174Z"},"accession":"S-EPMC8780672","cross_references":{"pubmed":["35054674"],"doi":["10.3390/polym14020267"]}}