{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Yi B"],"funding":["Breast Cancer Research Foundation","U.S. Department of Health & Human Services | NIH | National Cancer Institute","NCI NIH HHS","Alabama"],"pagination":["268-279"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8781987"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["41(2)"],"pubmed_abstract":["MicroRNA-3662 (miR-3662) is minimally expressed in normal human tissues but is highly expressed in all types of cancers, including breast cancer. As determined with The Cancer Genome Atlas dataset, miR-3662 expression is higher in triple-negative breast cancers (TNBCs) and African American breast cancers than in other breast cancer types. However, the functional role of miR-3662 remains a topic of debate. Here, we found that inhibition or knockout of endogenous, mature miR-3662 in TNBC cells suppresses proliferation and migration in vitro and tumor growth and metastasis in vivo. Functional analysis revealed that, for TNBC cells, knockout of miR-3662 reduces the activation of Wnt/β-catenin signaling. Furthermore, using CRISPR-mediated miR-3662 activation and repression, dual-luciferase assa"],"journal":["Oncogene"],"pubmed_title":["CRISPR interference and activation of the microRNA-3662-HBP1 axis control progression of triple-negative breast cancer."],"pmcid":["PMC8781987"],"funding_grant_id":["R56 CA223077","CA223077","R01 CA242917","R01 CA238273","CA242917","P30 CA013148","CA238273"],"pubmed_authors":["Liu Z","Stringer-Reasor E","Wang S","Bae S","Wei S","Yi B","Liu R","Wang X","Zhang C","Li M","Gao S","Wang L"],"additional_accession":[]},"is_claimable":false,"name":"CRISPR interference and activation of the microRNA-3662-HBP1 axis control progression of triple-negative breast cancer.","description":"MicroRNA-3662 (miR-3662) is minimally expressed in normal human tissues but is highly expressed in all types of cancers, including breast cancer. As determined with The Cancer Genome Atlas dataset, miR-3662 expression is higher in triple-negative breast cancers (TNBCs) and African American breast cancers than in other breast cancer types. However, the functional role of miR-3662 remains a topic of debate. Here, we found that inhibition or knockout of endogenous, mature miR-3662 in TNBC cells suppresses proliferation and migration in vitro and tumor growth and metastasis in vivo. Functional analysis revealed that, for TNBC cells, knockout of miR-3662 reduces the activation of Wnt/β-catenin signaling. Furthermore, using CRISPR-mediated miR-3662 activation and repression, dual-luciferase assa","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Jan","modification":"2026-05-10T02:11:15.142Z","creation":"2024-11-09T18:56:31.185Z"},"accession":"S-EPMC8781987","cross_references":{"pubmed":["34728806"],"doi":["10.1038/s41388-021-02089-6"]}}