biostudies-literatureYi BBreast Cancer Research FoundationU.S. Department of Health & Human Services | NIH | National Cancer InstituteNCI NIH HHSAlabama268-279https://www.ebi.ac.uk/biostudies/studies/S-EPMC8781987biostudies-literatureUnknown41(2)MicroRNA-3662 (miR-3662) is minimally expressed in normal human tissues but is highly expressed in all types of cancers, including breast cancer. As determined with The Cancer Genome Atlas dataset, miR-3662 expression is higher in triple-negative breast cancers (TNBCs) and African American breast cancers than in other breast cancer types. However, the functional role of miR-3662 remains a topic of debate. Here, we found that inhibition or knockout of endogenous, mature miR-3662 in TNBC cells suppresses proliferation and migration in vitro and tumor growth and metastasis in vivo. Functional analysis revealed that, for TNBC cells, knockout of miR-3662 reduces the activation of Wnt/β-catenin signaling. Furthermore, using CRISPR-mediated miR-3662 activation and repression, dual-luciferase assaOncogeneCRISPR interference and activation of the microRNA-3662-HBP1 axis control progression of triple-negative breast cancer.PMC8781987R56 CA223077CA223077R01 CA242917R01 CA238273CA242917P30 CA013148CA238273Liu ZStringer-Reasor EWang SBae SWei SYi BLiu RWang XZhang CLi MGao SWang LfalseCRISPR interference and activation of the microRNA-3662-HBP1 axis control progression of triple-negative breast cancer.MicroRNA-3662 (miR-3662) is minimally expressed in normal human tissues but is highly expressed in all types of cancers, including breast cancer. As determined with The Cancer Genome Atlas dataset, miR-3662 expression is higher in triple-negative breast cancers (TNBCs) and African American breast cancers than in other breast cancer types. However, the functional role of miR-3662 remains a topic of debate. Here, we found that inhibition or knockout of endogenous, mature miR-3662 in TNBC cells suppresses proliferation and migration in vitro and tumor growth and metastasis in vivo. Functional analysis revealed that, for TNBC cells, knockout of miR-3662 reduces the activation of Wnt/β-catenin signaling. Furthermore, using CRISPR-mediated miR-3662 activation and repression, dual-luciferase assa2022-01-01T00:00:00Z2022 Jan2026-05-10T02:11:15.142Z2024-11-09T18:56:31.185ZS-EPMC87819873472880610.1038/s41388-021-02089-6