{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Sachs L"],"funding":["Bundesministerium für Bildung und Forschung (Federal Ministry of Education and Research)","Deutsche Forschungsgemeinschaft (German Research Foundation)"],"pagination":["86"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8782918"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["5(1)"],"pubmed_abstract":["Inherited platelet disorders affecting the human platelet cytoskeleton result in increased bleeding risk. However, deciphering their impact on cytoskeleton-dependent intrinsic biomechanics of platelets remains challenging and represents an unmet need from a diagnostic and prognostic perspective. It is currently unclear whether ex vivo anticoagulants used during collection of peripheral blood impact the mechanophenotype of cellular components of blood. Using unbiased, high-throughput functional mechanophenotyping of single human platelets by real-time deformability cytometry, we found that ex vivo anticoagulants are a critical pre-analytical variable that differentially influences platelet deformation, their size, and functional response to agonists by altering the cytoskeleton. We applied "],"journal":["Communications biology"],"pubmed_title":["Ex vivo anticoagulants affect human blood platelet biomechanics with implications for high-throughput functional mechanophenotyping."],"pmcid":["PMC8782918"],"funding_grant_id":["03Z22CN11","374031971-CRC/TR 240"],"pubmed_authors":["Sachs L","Palankar R","Otto O","Wesche J","Lenkeit L","Greinacher A","Bender M"],"additional_accession":[]},"is_claimable":false,"name":"Ex vivo anticoagulants affect human blood platelet biomechanics with implications for high-throughput functional mechanophenotyping.","description":"Inherited platelet disorders affecting the human platelet cytoskeleton result in increased bleeding risk. However, deciphering their impact on cytoskeleton-dependent intrinsic biomechanics of platelets remains challenging and represents an unmet need from a diagnostic and prognostic perspective. It is currently unclear whether ex vivo anticoagulants used during collection of peripheral blood impact the mechanophenotype of cellular components of blood. Using unbiased, high-throughput functional mechanophenotyping of single human platelets by real-time deformability cytometry, we found that ex vivo anticoagulants are a critical pre-analytical variable that differentially influences platelet deformation, their size, and functional response to agonists by altering the cytoskeleton. We applied ","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Jan","modification":"2026-07-14T15:01:45.885Z","creation":"2025-04-06T04:01:59.433Z"},"accession":"S-EPMC8782918","cross_references":{"pubmed":["35064207"],"doi":["10.1038/s42003-021-02982-6"]}}