biostudies-literatureSun LNatural Science Foundation of Tianjin Municipal Science and Technology CommissionNational Natural Science Foundation of ChinaTianjin Research Innovation Project for Postgraduate Students for supporte2104051https://www.ebi.ac.uk/biostudies/studies/S-EPMC8787384biostudies-literatureUnknown9(3)Toll-like receptor (TLR) activation in macrophages plays a critical role in the pathogenesis of acute lung injury (ALI). While TLR inhibition is a promising strategy to control the overwhelming inflammation in ALI, there still lacks effective TLR inhibitors for clinical uses to date. A unique class of peptide-coated gold nanoparticles (GNPs) is previously discovered, which effectively inhibited TLR signaling and protected mice from lipopolysaccharide (LPS)-induced ALI. To fast translate such a discovery into potential clinical applicable nanotherapeutics, herein an elegant strategy of "nano-enabled drug repurposing" with "nano-targeting" is introduced to empower the existing drugs for new uses. Combining transcriptome sequencing with Connectivity Map analysis, it is identified that the proAdvanced science (Weinheim, Baden-Wurttemberg, Germany)Nano-Enabled Reposition of Proton Pump Inhibitors for TLR Inhibition: Toward A New Targeted Nanotherapy for Acute Lung Injury.PMC8787384819715498177007020JCYBJC000402020YJSB136Sun LLiu XYang HLiu YMa AWang RFung SYSaferali AMa HGao WGong JTurvey SEfalseNano-Enabled Reposition of Proton Pump Inhibitors for TLR Inhibition: Toward A New Targeted Nanotherapy for Acute Lung Injury.Toll-like receptor (TLR) activation in macrophages plays a critical role in the pathogenesis of acute lung injury (ALI). While TLR inhibition is a promising strategy to control the overwhelming inflammation in ALI, there still lacks effective TLR inhibitors for clinical uses to date. A unique class of peptide-coated gold nanoparticles (GNPs) is previously discovered, which effectively inhibited TLR signaling and protected mice from lipopolysaccharide (LPS)-induced ALI. To fast translate such a discovery into potential clinical applicable nanotherapeutics, herein an elegant strategy of "nano-enabled drug repurposing" with "nano-targeting" is introduced to empower the existing drugs for new uses. Combining transcriptome sequencing with Connectivity Map analysis, it is identified that the pro2022-01-01T00:00:00Z2022 Jan2026-05-09T04:28:56.372Z2022-02-11T15:46:41.64ZS-EPMC87873843481663010.1002/advs.202104051