biostudies-literatureHong LSwiss National Science FoundationProject of Tianjin Science and Technology PlanOpen Project Program of State Key Laboratory of Food Nutrition and Safety, Tianjin University of Science and TechnologyNational Natural Science Foundation of China51https://www.ebi.ac.uk/biostudies/studies/S-EPMC8787952biostudies-literatureUnknown20(1)In this study, a thermo-sensitive molecularly imprinted fluorescence sensor was developed for the specific detection of β-Lactoglobulin (β-LG) allergen in milk products. The metal-organic frameworks (MIL-100) with a high specific surface area was coated on the surface of upconversion micro-particles (UCMPs). As the core, an imprinted polymer layer allowing for swelling and shrinking with response to temperature was prepared, which exhibited high adsorption and mass transfer capabilities for β-LG allergen. The fluorescence intensity of UCMPs@MIL-100@MIP decreased linearly with the concentration of β-LG in the range of 0.1-0.8 mg mL^-1, and the limit of detection was 0.043 mg mL^-1. The imprinting factor reached 3.415, which indicated that excellent specificity of the UCMJournal of nanobiotechnologyA UCMPs@MIL-100 based thermo-sensitive molecularly imprinted fluorescence sensor for effective detection of β-lactoglobulin allergen in milk products.PMC8787952No. SKLFNS-KF-202115No. 20ZYJDJC00040No. 31972147202115Hong LYang XYang JLiu KXie XWang SPan MfalseA UCMPs@MIL-100 based thermo-sensitive molecularly imprinted fluorescence sensor for effective detection of β-lactoglobulin allergen in milk products.In this study, a thermo-sensitive molecularly imprinted fluorescence sensor was developed for the specific detection of β-Lactoglobulin (β-LG) allergen in milk products. The metal-organic frameworks (MIL-100) with a high specific surface area was coated on the surface of upconversion micro-particles (UCMPs). As the core, an imprinted polymer layer allowing for swelling and shrinking with response to temperature was prepared, which exhibited high adsorption and mass transfer capabilities for β-LG allergen. The fluorescence intensity of UCMPs@MIL-100@MIP decreased linearly with the concentration of β-LG in the range of 0.1-0.8 mg mL^-1, and the limit of detection was 0.043 mg mL^-1. The imprinting factor reached 3.415, which indicated that excellent specificity of the UCM2022-01-01T00:00:00Z2022 Jan2025-04-04T08:34:18.353Z2025-04-04T08:34:18.353ZS-EPMC87879523507848010.1186/s12951-022-01258-3