{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Wurtz M"],"funding":["Deutsche Forschungsgemeinschaft"],"pagination":["473"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8789826"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["13(1)"],"pubmed_abstract":["The gamma-tubulin ring complex (γ-TuRC) is the principal microtubule nucleation template in vertebrates. Recent cryo-EM reconstructions visualized the intricate quaternary structure of the γ-TuRC, containing more than thirty subunits, raising fundamental questions about γ-TuRC assembly and the role of actin as an integral part of the complex. Here, we reveal the structural mechanism underlying modular γ-TuRC assembly and identify a functional role of actin in microtubule nucleation. During γ-TuRC assembly, a GCP6-stabilized core comprising GCP2-3-4-5-4-6 is expanded by stepwise recruitment, selective stabilization and conformational locking of four pre-formed GCP2-GCP3 units. Formation of the lumenal bridge specifies incorporation of the terminal GCP2-GCP3 unit and thereby leads to closure"],"journal":["Nature communications"],"pubmed_title":["Modular assembly of the principal microtubule nucleator γ-TuRC."],"pmcid":["PMC8789826"],"funding_grant_id":["DFG Schi 295/4-4","DFG PF 963/1-4"],"pubmed_authors":["Rahadian AS","Atorino ES","Pfeffer S","Wurtz M","Bohler A","Schiebel E","Zupa E","Eustermann S","Neuner A","Vermeulen BJA","Tonon G"],"additional_accession":[]},"is_claimable":false,"name":"Modular assembly of the principal microtubule nucleator γ-TuRC.","description":"The gamma-tubulin ring complex (γ-TuRC) is the principal microtubule nucleation template in vertebrates. Recent cryo-EM reconstructions visualized the intricate quaternary structure of the γ-TuRC, containing more than thirty subunits, raising fundamental questions about γ-TuRC assembly and the role of actin as an integral part of the complex. Here, we reveal the structural mechanism underlying modular γ-TuRC assembly and identify a functional role of actin in microtubule nucleation. During γ-TuRC assembly, a GCP6-stabilized core comprising GCP2-3-4-5-4-6 is expanded by stepwise recruitment, selective stabilization and conformational locking of four pre-formed GCP2-GCP3 units. Formation of the lumenal bridge specifies incorporation of the terminal GCP2-GCP3 unit and thereby leads to closure","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Jan","modification":"2026-05-30T20:56:20.209Z","creation":"2025-04-04T10:02:11.28Z"},"accession":"S-EPMC8789826","cross_references":{"pubmed":["35078983"],"doi":["10.1038/s41467-022-28079-0"]}}