{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Laffoon SB"],"funding":["NCRR NIH HHS","Alzheimer's Drug Discovery Foundation","National Health and Medical Research Council","National Institutes of Health","NIH HHS"],"pagination":["2"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8790928"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["20(1)"],"pubmed_abstract":["Background
The Australian Imaging and Biomarker Lifestyle (AIBL) study of aging is designed to aid the discovery of biomarkers. The current study aimed to discover differentially expressed plasma proteins that could yield a blood-based screening tool for Alzheimer's disease.Methods
The concentration of proteins in plasma covers a vast range of 12 orders of magnitude. Therefore, to search for medium to low abundant biomarkers and elucidate mechanisms of AD, we immuno-depleted the most abundant plasma proteins and pre-fractionated the remaining proteins by HPLC, prior to two-dimensional gel electrophoresis. The relative levels of approximately 3400 protein species resolved on the 2D gels were compared using in-gel differential analysis with spectrally resolved fluorescent pro"],"journal":["Proteome science"],"pubmed_title":["Analysis of plasma proteins using 2D gels and novel fluorescent probes: in search of blood based biomarkers for Alzheimer's disease."],"pmcid":["PMC8790928"],"funding_grant_id":["1164692","P20 RR024237","1P20RR024237","5R42RR021790","1138673","R42 RR021790"],"pubmed_authors":["Roberts AM","Trounson B","Ames D","Roberts BR","Bush AI","Reeves BD","Vance JA","Masters CL","Pertile KK","Doecke JD","Grieco PA","Laffoon SB","Fowler CJ","Martins R","Dratz EA","Rumble RL"],"additional_accession":[]},"is_claimable":false,"name":"Analysis of plasma proteins using 2D gels and novel fluorescent probes: in search of blood based biomarkers for Alzheimer's disease.","description":"Background
The Australian Imaging and Biomarker Lifestyle (AIBL) study of aging is designed to aid the discovery of biomarkers. The current study aimed to discover differentially expressed plasma proteins that could yield a blood-based screening tool for Alzheimer's disease.Methods
The concentration of proteins in plasma covers a vast range of 12 orders of magnitude. Therefore, to search for medium to low abundant biomarkers and elucidate mechanisms of AD, we immuno-depleted the most abundant plasma proteins and pre-fractionated the remaining proteins by HPLC, prior to two-dimensional gel electrophoresis. The relative levels of approximately 3400 protein species resolved on the 2D gels were compared using in-gel differential analysis with spectrally resolved fluorescent pro","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Jan","modification":"2026-05-08T08:38:08.888Z","creation":"2022-02-11T15:52:48.392Z"},"accession":"S-EPMC8790928","cross_references":{"pubmed":["35081972"],"doi":["10.1186/s12953-021-00185-9"]}}