biostudies-literatureLim DJNIDDK NIH HHSNIAID NIH HHSNHLBI NIH HHS181-190https://www.ebi.ac.uk/biostudies/studies/S-EPMC8792167biostudies-literatureUnknown12(2)<h4>Background</h4>Hypoxia due to closure at the ostiomeatal complex is widely considered one of the major pathogenic mechanisms leading to chronic inflammation in chronic rhinosinusitis (CRS). The objective of this study was to develop and characterize an oxygen-generating biomaterial (OGB) as an innovative treatment strategy for CRS.<h4>Methods</h4>An OGB was fabricated by coating hydrophobic beeswax (BW, 15mg or 30mg) on the surface of calcium peroxide - catalase complex (CPO-CA, 30mg) and characterized using scanning electron microscopy (SEM). In vitro releases of both oxygen and hydrogen peroxide (H₂ O₂ ) were spectrophotometrically quantified, and cytotoxicity in human sinonasal epithelial cells (HSNECs) was evaluated. The influence of OGB on transepithelial Cl<International forum of allergy & rhinologyIn vitro evaluation of a novel oxygen-generating biomaterial for chronic rhinosinusitis therapy.PMC8792167K08 AI146220R01 HL133006P30 DK072482West JMAyinala SWoodworth BAGrayson JWCho DYLim DJSkinner DZhang SfalseIn vitro evaluation of a novel oxygen-generating biomaterial for chronic rhinosinusitis therapy.<h4>Background</h4>Hypoxia due to closure at the ostiomeatal complex is widely considered one of the major pathogenic mechanisms leading to chronic inflammation in chronic rhinosinusitis (CRS). The objective of this study was to develop and characterize an oxygen-generating biomaterial (OGB) as an innovative treatment strategy for CRS.<h4>Methods</h4>An OGB was fabricated by coating hydrophobic beeswax (BW, 15mg or 30mg) on the surface of calcium peroxide - catalase complex (CPO-CA, 30mg) and characterized using scanning electron microscopy (SEM). In vitro releases of both oxygen and hydrogen peroxide (H₂ O₂ ) were spectrophotometrically quantified, and cytotoxicity in human sinonasal epithelial cells (HSNECs) was evaluated. The influence of OGB on transepithelial Cl<2022-01-01T00:00:00Z2022 Feb2025-04-05T09:05:06.828Z2025-04-05T09:05:06.828ZS-EPMC87921673444837210.1002/alr.22875