{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Master A"],"funding":["Stony Brook University","NEI NIH HHS","National Institutes of Health"],"pagination":["41-50"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8792174"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["47(1)"],"pubmed_abstract":["<h4>Purpose</h4>To develop a more efficient impression cytology (IC) method for the transfer of ocular surface cells onto glass microscope slides for cytochemical, immunocytochemical, and immunofluorescence studies.<h4>Methods</h4>Cells are lifted off the ocular surface with a mixed cellulose ester membrane and then firmly attached to a glass slide using a novel triblock copolymer comprised of collagen type I, polyethylenimine and poly-L-lysine (CPP), and crosslinking cells and glass slide by heating and cooling. The membrane is removed intact after softening it with a butanol/ethanol solution. Transfer of cells is complete in about 10-15 minutes and is ready for staining. The efficiency of our cell transfer method was compared to current methods based on poly-L-lysine and albumin paste.<h"],"journal":["Current eye research"],"pubmed_title":["An Improved Ocular Impression Cytology Method: Quantitative Cell Transfer to Microscope Slides Using a Novel Polymer."],"pmcid":["PMC8792174"],"funding_grant_id":["R44 EY031193","R44EY031193"],"pubmed_authors":["Rigas B","Master A","Huang W","Huang L","Honkanen R"],"additional_accession":[]},"is_claimable":false,"name":"An Improved Ocular Impression Cytology Method: Quantitative Cell Transfer to Microscope Slides Using a Novel Polymer.","description":"<h4>Purpose</h4>To develop a more efficient impression cytology (IC) method for the transfer of ocular surface cells onto glass microscope slides for cytochemical, immunocytochemical, and immunofluorescence studies.<h4>Methods</h4>Cells are lifted off the ocular surface with a mixed cellulose ester membrane and then firmly attached to a glass slide using a novel triblock copolymer comprised of collagen type I, polyethylenimine and poly-L-lysine (CPP), and crosslinking cells and glass slide by heating and cooling. The membrane is removed intact after softening it with a butanol/ethanol solution. Transfer of cells is complete in about 10-15 minutes and is ready for staining. The efficiency of our cell transfer method was compared to current methods based on poly-L-lysine and albumin paste.<h","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Jan","modification":"2025-08-30T03:08:16.867Z","creation":"2025-04-06T09:40:55.602Z"},"accession":"S-EPMC8792174","cross_references":{"pubmed":["34841993"],"doi":["10.1080/02713683.2021.1951300"]}}