biostudies-literatureMaster AStony Brook UniversityNEI NIH HHSNational Institutes of Health41-50https://www.ebi.ac.uk/biostudies/studies/S-EPMC8792174biostudies-literatureUnknown47(1)<h4>Purpose</h4>To develop a more efficient impression cytology (IC) method for the transfer of ocular surface cells onto glass microscope slides for cytochemical, immunocytochemical, and immunofluorescence studies.<h4>Methods</h4>Cells are lifted off the ocular surface with a mixed cellulose ester membrane and then firmly attached to a glass slide using a novel triblock copolymer comprised of collagen type I, polyethylenimine and poly-L-lysine (CPP), and crosslinking cells and glass slide by heating and cooling. The membrane is removed intact after softening it with a butanol/ethanol solution. Transfer of cells is complete in about 10-15 minutes and is ready for staining. The efficiency of our cell transfer method was compared to current methods based on poly-L-lysine and albumin paste.<hCurrent eye researchAn Improved Ocular Impression Cytology Method: Quantitative Cell Transfer to Microscope Slides Using a Novel Polymer.PMC8792174R44 EY031193R44EY031193Rigas BMaster AHuang WHuang LHonkanen RfalseAn Improved Ocular Impression Cytology Method: Quantitative Cell Transfer to Microscope Slides Using a Novel Polymer.<h4>Purpose</h4>To develop a more efficient impression cytology (IC) method for the transfer of ocular surface cells onto glass microscope slides for cytochemical, immunocytochemical, and immunofluorescence studies.<h4>Methods</h4>Cells are lifted off the ocular surface with a mixed cellulose ester membrane and then firmly attached to a glass slide using a novel triblock copolymer comprised of collagen type I, polyethylenimine and poly-L-lysine (CPP), and crosslinking cells and glass slide by heating and cooling. The membrane is removed intact after softening it with a butanol/ethanol solution. Transfer of cells is complete in about 10-15 minutes and is ready for staining. The efficiency of our cell transfer method was compared to current methods based on poly-L-lysine and albumin paste.<h2022-01-01T00:00:00Z2022 Jan2025-08-30T03:08:16.867Z2025-04-06T09:40:55.602ZS-EPMC87921743484199310.1080/02713683.2021.1951300