{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Yu W"],"funding":["Cystic Fibrosis Foundation","NHLBI NIH HHS","HHS | NIH | National Heart, Lung, and Blood Institute","NIGMS NIH HHS"],"pagination":["e2119759119"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8794846"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["119(4)"],"pubmed_abstract":["Submucosal glands (SMGs) protect lungs but can also contribute to disease. For example, in cystic fibrosis (CF), SMGs produce abnormal mucus that disrupts mucociliary transport. CF is an ion transport disease, yet knowledge of the ion transporters expressed by SMG acini, which produce mucus, and SMG ducts that carry it to the airway lumen is limited. Therefore, we isolated SMGs from newborn pigs and used single-cell messenger RNA sequencing, immunohistochemistry, and in situ hybridization to identify cell types, gene expression, and spatial distribution. Cell types and transcript levels were the same in non-CF and CF SMGs, suggesting that loss of epithelial anion secretion rather than an intrinsic cell defect causes CF mucus abnormalities. Gene signatures of acinar mucous and acinar serous"],"journal":["Proceedings of the National Academy of Sciences of the United States of America"],"pubmed_title":["Cellular and molecular architecture of submucosal glands in wild-type and cystic fibrosis pigs."],"pmcid":["PMC8794846"],"funding_grant_id":["P01 HL091842","HL091842","P01 HL152960","T32 GM139776","P01 HL051670","HL051670","RDP"],"pubmed_authors":["Moninger TO","Welsh MJ","Jain A","Xie Y","Zarei K","Powers LS","Yu W","Pezzulo AA","Stoltz DA","Thurman AL"],"additional_accession":[]},"is_claimable":false,"name":"Cellular and molecular architecture of submucosal glands in wild-type and cystic fibrosis pigs.","description":"Submucosal glands (SMGs) protect lungs but can also contribute to disease. For example, in cystic fibrosis (CF), SMGs produce abnormal mucus that disrupts mucociliary transport. CF is an ion transport disease, yet knowledge of the ion transporters expressed by SMG acini, which produce mucus, and SMG ducts that carry it to the airway lumen is limited. Therefore, we isolated SMGs from newborn pigs and used single-cell messenger RNA sequencing, immunohistochemistry, and in situ hybridization to identify cell types, gene expression, and spatial distribution. Cell types and transcript levels were the same in non-CF and CF SMGs, suggesting that loss of epithelial anion secretion rather than an intrinsic cell defect causes CF mucus abnormalities. Gene signatures of acinar mucous and acinar serous","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Jan","modification":"2025-04-22T08:34:33.294Z","creation":"2025-04-05T22:35:12.627Z"},"accession":"S-EPMC8794846","cross_references":{"pubmed":["35046051"],"doi":["10.1073/pnas.2119759119"]}}