{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Stachelek P"],"funding":["Biotechnology and Biological Sciences Research Council","Engineering and Physical Sciences Research Council"],"pagination":["553"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8795401"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["13(1)"],"pubmed_abstract":["The molecular machinery of life is founded on chiral building blocks, but no experimental technique is currently available to distinguish or monitor chiral systems in live cell bio-imaging studies. Luminescent chiral molecules encode a unique optical fingerprint within emitted circularly polarized light (CPL) carrying information about the molecular environment, conformation, and binding state. Here, we present a CPL Laser Scanning Confocal Microscope (CPL-LSCM) capable of simultaneous chiroptical contrast based live-cell imaging of endogenous and engineered CPL-active cellular probes. Further, we demonstrate that CPL-active probes can be activated using two-photon excitation, with complete CPL spectrum recovery. The combination of these two milestone results empowers the multidisciplinary"],"journal":["Nature communications"],"pubmed_title":["Circularly polarised luminescence laser scanning confocal microscopy to study live cell chiral molecular interactions."],"pmcid":["PMC8795401"],"funding_grant_id":["EP/I010319/1","BB/T009268/1","BB/T009268/2","BB/S017615/1","EP/P025013/1"],"pubmed_authors":["Stachelek P","MacKenzie L","Pal R","Parker D"],"additional_accession":[]},"is_claimable":false,"name":"Circularly polarised luminescence laser scanning confocal microscopy to study live cell chiral molecular interactions.","description":"The molecular machinery of life is founded on chiral building blocks, but no experimental technique is currently available to distinguish or monitor chiral systems in live cell bio-imaging studies. Luminescent chiral molecules encode a unique optical fingerprint within emitted circularly polarized light (CPL) carrying information about the molecular environment, conformation, and binding state. Here, we present a CPL Laser Scanning Confocal Microscope (CPL-LSCM) capable of simultaneous chiroptical contrast based live-cell imaging of endogenous and engineered CPL-active cellular probes. Further, we demonstrate that CPL-active probes can be activated using two-photon excitation, with complete CPL spectrum recovery. The combination of these two milestone results empowers the multidisciplinary","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Jan","modification":"2026-07-16T16:06:29.814Z","creation":"2025-04-05T13:23:40.106Z"},"accession":"S-EPMC8795401","cross_references":{"pubmed":["35087047"],"doi":["10.1038/s41467-022-28220-z"]}}