biostudies-literatureLoeser RFArthritis FoundationNational Institute of Arthritis and Musculoskeletal and Skin DiseasesNational Center for Advancing Translational SciencesNCATS NIH HHSNCCDPHP CDC HHSCrohn's & Colitis Foundation of AmericaNational Institute of Diabetes and Digestive and Kidney DiseasesNIH Office of the DirectorOffice of the Director, NIHCenters for Disease Control and PreventionNIDDK NIH HHSNIAMS NIH HHSNIH HHSNutrition Obesity Resource Core of the National Institute of Diabetes and Digestive and Kidney DiseasesCDC HHS227-236https://www.ebi.ac.uk/biostudies/studies/S-EPMC8795472biostudies-literatureUnknown74(2)<h4>Objective</h4>To test the hypothesis that an altered gut microbiota (dysbiosis) plays a role in obesity-associated osteoarthritis (OA).<h4>Methods</h4>Stool and blood samples were collected from 92 participants with a body mass index (BMI) ≥30 kg/m² , recruited from the Johnston County Osteoarthritis Project. OA patients (n = 50) had hand and knee OA (Kellgren/Lawrence [K/L] grade ≥2 or arthroplasty). Controls (n = 42) had no hand OA and a K/L grade of 0-1 for the knees. Compositional analysis of stool samples was carried out by 16S ribosomal RNA amplicon sequencing. Alpha- and beta-diversity and differences in taxa relative abundances were determined. Blood samples were used for multiplex cytokine analysis and measures of lipopolysaccharide (LPS) and LPS binding protein. GeArthritis & rheumatology (Hoboken, N.J.)Association of Increased Serum Lipopolysaccharide, But Not Microbial Dysbiosis, With Obesity-Related Osteoarthritis.PMC8795472UL1-TR-002489P30 DK056350P30-DK-034987UL1 TR002489P30-DK-056350U01‐DP‐006266P40 OD010995U01-DP-006266U01 DP006266P40‐OD‐010995P30‐DK‐056350718285P40-OD-010995P30‐AR‐072580P30 DK034987UL1‐TR‐002489P30-AR-072580P30‐DK‐034987P30 AR072580Arbeeva LJordan JMAzcarate-Peril MAKelley KLongobardi LRenner JBCarroll IMCui YSartor RBSun SFodor AANelson AEUlici VStewart DASumner SJLoeser RFfalseAssociation of Increased Serum Lipopolysaccharide, But Not Microbial Dysbiosis, With Obesity-Related Osteoarthritis.<h4>Objective</h4>To test the hypothesis that an altered gut microbiota (dysbiosis) plays a role in obesity-associated osteoarthritis (OA).<h4>Methods</h4>Stool and blood samples were collected from 92 participants with a body mass index (BMI) ≥30 kg/m² , recruited from the Johnston County Osteoarthritis Project. OA patients (n = 50) had hand and knee OA (Kellgren/Lawrence [K/L] grade ≥2 or arthroplasty). Controls (n = 42) had no hand OA and a K/L grade of 0-1 for the knees. Compositional analysis of stool samples was carried out by 16S ribosomal RNA amplicon sequencing. Alpha- and beta-diversity and differences in taxa relative abundances were determined. Blood samples were used for multiplex cytokine analysis and measures of lipopolysaccharide (LPS) and LPS binding protein. Ge2022-01-01T00:00:00Z2022 Feb2025-04-05T09:03:13.597Z2025-04-05T09:03:13.597ZS-EPMC87954723442391810.1002/art.41955