{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["20(1)"],"submitter":["Tzounakas VL"],"pubmed_abstract":["<h4>Background</h4>Proteasomes are proteolytic complexes with prominent roles in the control of protein homeostasis and cellular viability. However, little is known about the effects of storage and glucose-6-phosphate dehydrogenase deficiency (G6PD<sup>-</sup>) on the activity and topology of red blood cell (RBC) proteasomes.<h4>Materials and methods</h4>We investigated the concentration (by GeLC-MS proteomics analysis and immunoblotting), activity (by using peptide substrates and proteasome inhibitors), and subcellular/extracellular distribution (following cell fractionation and isolation of extracellular vesicles, respectively) of RBC proteasomes in fresh blood and RBCs from control and G6PD<sup>-</sup> donors following storage in leukoreduced units. RBC proteasome activity was also test"],"journal":["Blood transfusion = Trasfusione del sangue"],"pagination":["27-39"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8796840"],"repository":["biostudies-literature"],"pubmed_title":["Red cell proteasome modulation by storage, redox metabolism and transfusion."],"pmcid":["PMC8796840"],"pubmed_authors":["Papassideri IS","D'Alessandro A","Anastasiadi AT","Dzieciatkowska M","Stamoulis K","Siourounis P","Kriebardis AG","Vergaki A","Karadimas DG","Tzounakas VL","Antonelou MH"],"additional_accession":[]},"is_claimable":false,"name":"Red cell proteasome modulation by storage, redox metabolism and transfusion.","description":"<h4>Background</h4>Proteasomes are proteolytic complexes with prominent roles in the control of protein homeostasis and cellular viability. However, little is known about the effects of storage and glucose-6-phosphate dehydrogenase deficiency (G6PD<sup>-</sup>) on the activity and topology of red blood cell (RBC) proteasomes.<h4>Materials and methods</h4>We investigated the concentration (by GeLC-MS proteomics analysis and immunoblotting), activity (by using peptide substrates and proteasome inhibitors), and subcellular/extracellular distribution (following cell fractionation and isolation of extracellular vesicles, respectively) of RBC proteasomes in fresh blood and RBCs from control and G6PD<sup>-</sup> donors following storage in leukoreduced units. RBC proteasome activity was also test","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Jan","modification":"2025-04-04T07:45:28.872Z","creation":"2025-04-04T07:45:28.872Z"},"accession":"S-EPMC8796840","cross_references":{"pubmed":["33263521"],"doi":["10.2450/2020.0179-20"]}}